Ilavenil Soundharrajan, Al-Dhabi Naif Abdullah, Srigopalram Srisesharam, Kim Young Ock, Agastian Paul, Baaru Rajasekhar, Choi Ki Choon, Arasu Mariadhas Valan, Park Chun Geon, Park Kyung Hun
Grassland and Forage Division, National Institute of Animal Science, RDA, Seonghwan-Eup, Cheonan-Si, Chungnam, 330801 Korea.
Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P. O. Box 2455, Riyadh, 11451 Saudi Arabia.
Proteome Sci. 2016 Sep 6;14(1):11. doi: 10.1186/s12953-016-0098-5. eCollection 2016.
Metal-organic frameworks (MOFs - MIL-101) are the most exciting, high profiled developments in nanotechnology in the last ten years, and it attracted considerable attention owing to their uniform nanoporosity, large surface area, outer-surface modification and in-pore functionality for tailoring the chemical properties of the material for anchoring specific guest moieties. MOF's have been particularly highlighted for their excellent gas storage and separation properties. Recently biomolecules-based MOF's were used as nanoencapsulators for antitumor and antiretroviral controlled drug delivery studies. However, usage of MOF material for removal of ionic detergent-SDS from biological samples has not been reported to date. Here, first time we demonstrate its novel applications in biological sample preparation for mass spectrometry analysis.
SDS removal using MIL-101 was assessed for proteomic analysis by mass spectrometry. We analysed removal of SDS from 0.5 % SDS solution alone, BSA mixture and HMEC cells lysate protein mixture. The removal of SDS by MIL-101 was confirmed by MALDI-TOF-MS and LC-MS techniques.
In an initial demonstration, SDS has removed effectively from 0.5 % SDS solution by MIL-101via its binding attraction with SDS. Further, the experiment also confirmed that MIL-101 strongly removed the SDS from BSA and cell lysate mixtures.
These results suggest that SDS removal by the MIL-101 method is a practical, simple and broad applicable in proteomic sample processing for MALDI-TOF-MS and LC-MS analysis.
金属有机框架材料（MOFs - MIL - 101）是过去十年中纳米技术领域最令人兴奋且备受瞩目的进展，因其具有均匀的纳米孔隙率、大表面积、外表面可修饰性以及孔内功能性，能够定制材料的化学性质以锚定特定客体部分，故而吸引了广泛关注。MOF材料因其出色的气体存储和分离性能而备受特别关注。最近，基于生物分子的MOF材料被用作纳米封装剂用于抗肿瘤和抗逆转录病毒药物的控释研究。然而，迄今为止尚未有关于使用MOF材料从生物样品中去除离子型去污剂十二烷基硫酸钠（SDS）的报道。在此，我们首次展示了其在用于质谱分析的生物样品制备中的新应用。
通过质谱法评估使用MIL - 101去除SDS用于蛋白质组学分析的效果。我们分析了从单独的0.5% SDS溶液、牛血清白蛋白（BSA）混合物以及人微血管内皮细胞（HMEC）裂解物蛋白质混合物中去除SDS的情况。通过基质辅助激光解吸电离飞行时间质谱（MALDI - TOF - MS）和液相色谱 - 质谱（LC - MS）技术确认了MIL - 101对SDS的去除。
在初步验证中，MIL - 101通过与SDS的结合吸引力有效地从0.5% SDS溶液中去除了SDS。此外，实验还证实MIL - 101能从BSA和细胞裂解物混合物中强力去除SDS。
这些结果表明，采用MIL - 101方法去除SDS在用于MALDI - TOF - MS和LC - MS分析的蛋白质组学样品处理中是实用、简便且具有广泛适用性的。
