Chongqing Key Laboratory for Disease Proteomics, State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burn Research, Southwest Hospital, The Third Military Medical University, Chongqing, 400038 China ; School of Environmental Air Security and Pollution Control Engineering, Jinan University, Guangzhou, China.
Chongqing Key Laboratory for Disease Proteomics, State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burn Research, Southwest Hospital, The Third Military Medical University, Chongqing, 400038 China ; The 421 Hospital, People's Liberation Army, Guangzhou, China.
Burns Trauma. 2014 Apr 6;2(2):76-83. doi: 10.4103/2321-3868.130191. eCollection 2014.
The formation of hypertrophic scars (HSs) is a fibroproliferative disorder of abnormal wound healing. HSs usually characterize excessive proliferation of fibroblasts, abnormal deposition of extracellular matrix (ECM) during wound healing, associated with cosmetic, functional, and psychological problems. Owing to the role of ECM proteins in scar formation, we comparatively analyzed matrix proteins secreted by normal skin fibroblasts (NSFs) and HS fibroblasts (HSFs). The acetone-extracted secreted proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and identified by mass spectrometry (MS). Based on Go annotation of MS data, the profiling of ECM proteins was established and scar-related proteins have been screened out. The functions of several ECM proteins identified by MS have been discussed, such as collagens I, VI, XII, fibronectin, decorin, lumican, and protein procollagen C endopeptidase enhancer 1 (PCPE-1). Among them, the MS result of PCPE-1 was supported by Western blotting that PCPE-1 from HSFs were significantly upregulated than that from NSFs. It is suggested that PCPE-1 could be a potential target for scar treatment. The exploration of scar related proteins may provide new perspectives on understanding the mechanism of scar formation and open a new way to scar treatment and prevention.
增生性瘢痕（HSs）的形成是一种异常愈合的纤维增生性疾病。HSs 通常表现为成纤维细胞过度增殖、细胞外基质（ECM）在愈合过程中的异常沉积，与美容、功能和心理问题有关。由于 ECM 蛋白在瘢痕形成中的作用，我们比较分析了正常皮肤成纤维细胞（NSFs）和 HS 成纤维细胞（HSFs）分泌的基质蛋白。用丙酮提取分泌的蛋白质，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分离，并用质谱法（MS）鉴定。根据 MS 数据的 GO 注释，建立了 ECM 蛋白图谱，并筛选出与瘢痕相关的蛋白。讨论了通过 MS 鉴定的几种 ECM 蛋白的功能，如胶原蛋白 I、VI、XII、纤维连接蛋白、饰胶蛋白聚糖、亮氨酸丰富蛋白聚糖和蛋白原胶原 C 端肽酶增强子 1（PCPE-1）。其中，PCPE-1 的 MS 结果通过 Western blot 得到支持，HSFs 中的 PCPE-1 明显高于 NSFs。这表明 PCPE-1 可能是瘢痕治疗的潜在靶点。对瘢痕相关蛋白的探索可能为理解瘢痕形成机制提供新的视角，并为瘢痕的治疗和预防开辟新途径。
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