De Pieri Andrea, Rochev Yury, Zeugolis Dimitrios I
Regenerative, Modular & Developmental Engineering Laboratory (REMODEL), Biomedical Sciences Building, National University of Ireland Galway (NUI Galway), Galway, Ireland.
Science Foundation Ireland (SFI) Centre for Research in Medical Devices (CÚRAM), Biomedical Sciences Building, National University of Ireland Galway (NUI Galway), Galway, Ireland.
NPJ Regen Med. 2021 Mar 29;6(1):18. doi: 10.1038/s41536-021-00133-3.
Cell-based scaffold-free therapies seek to develop in vitro organotypic three-dimensional (3D) tissue-like surrogates, capitalising upon the inherent capacity of cells to create tissues with efficiency and sophistication that is still unparalleled by human-made devices. Although automation systems have been realised and (some) success stories have been witnessed over the years in clinical and commercial arenas, in vitro organogenesis is far from becoming a standard way of care. This limited technology transfer is largely attributed to scalability-associated costs, considering that the development of a borderline 3D implantable device requires very high number of functional cells and prolonged ex vivo culture periods. Herein, we critically discuss advancements and shortfalls of scaffold-free cell-based tissue engineering strategies, along with pioneering concepts that have the potential to transform regenerative and reparative medicine.
基于细胞的无支架疗法旨在开发体外器官型三维（3D）组织样替代物，利用细胞固有的能力高效且精细地构建组织，这仍然是人造设备无法比拟的。尽管多年来已经实现了自动化系统，并且在临床和商业领域也见证了（一些）成功案例，但体外器官发生远未成为一种标准的护理方式。考虑到开发一种临界的3D可植入装置需要大量的功能细胞和较长的体外培养时间，这种有限的技术转移在很大程度上归因于与可扩展性相关的成本。在此，我们批判性地讨论了无支架细胞基组织工程策略的进展和不足，以及有可能改变再生医学和修复医学的开创性概念。
