Rodil Andrea, Laca Amanda, Paredes Benjamín, Rendueles Manuel, Meana Álvaro, Díaz Mario
Dept. of Chemical Engineering and Environmental Technology, University of Oviedo, C/Julián Clavería s/n, Oviedo, 33071, Spain.
Community Center of Blood and Tissues of the Princedom of Asturias, C/Emilio Rodriguez Vigil s/n, Oviedo, 33006, Spain.
Biotechnol Prog. 2016 Nov;32(6):1577-1583. doi: 10.1002/btpr.2364. Epub 2016 Sep 21.
New biomaterials prepared from egg yolk and its main fractions (plasma and granules) have been developed for use in tissue engineering. Protein gels obtained via transglutaminase cross-linking were characterized by rheometry, texturometry and scanning electron microscopy. All the gels exhibited suitable physical and mechanical characteristics for use as potential biomaterials in skin regeneration. Specifically, results showed that these materials presented a compact, uniform structure, with granular gel being found to be the most resistant as well as the most elastic material. Accordingly, these gels were subsequently evaluated as scaffolds for murine fibroblast growth. The best results were obtained with granule gels. Not only adhesion and cell growth were detected when using these gels, but also continuous coatings of cells growing on their surface. These findings can be attributed to the higher protein content of this fraction and to the particular structure of its proteins. Thus, granules have proved to be an interesting potential raw material for scaffold development. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1577-1583, 2016.
由蛋黄及其主要成分（血浆和颗粒）制备的新型生物材料已被开发用于组织工程。通过转谷氨酰胺酶交联获得的蛋白质凝胶通过流变学、质地测定法和扫描电子显微镜进行表征。所有凝胶均表现出适合作为皮肤再生潜在生物材料的物理和机械特性。具体而言，结果表明这些材料呈现出致密、均匀的结构，发现颗粒凝胶是最具抗性且最具弹性的材料。因此，这些凝胶随后被评估为小鼠成纤维细胞生长的支架。颗粒凝胶获得了最佳结果。使用这些凝胶时不仅检测到细胞粘附和生长，还检测到在其表面生长的细胞连续涂层。这些发现可归因于该部分较高的蛋白质含量及其蛋白质的特殊结构。因此，颗粒已被证明是用于支架开发的一种有趣的潜在原材料。© 2016美国化学工程师学会生物技术进展，32:1577 - 1583，2016。
