Department of Chemical Engineering and Environment Technology, Faculty of Chemistry, University of Oviedo, C/Julián Clavería s/n, 33071 Oviedo, Spain.
Blood and Tissue Community Center of Asturias, Spain; Centro de Investigación Biomédica en Red en Enfermedades Raras (CIBERER), ISCIII, Madrid, Spain; Instituto de Investigación Sanitaria de la Fundación Jimenez Díaz (IIS-FJD), Madrid, Spain.
J Mech Behav Biomed Mater. 2019 Jan;89:107-113. doi: 10.1016/j.jmbbm.2018.09.015. Epub 2018 Sep 13.
Tissue engineering is one of the fields of clinical medicine that has forged ahead in recent years, especially because of its role as a potential alternative to organ transplantation. The main aim of this study has been the development of biocompatible materials to form extracellular matrix (ECM) structures in order to provide the necessary conditions for the settlement, proliferation and differentiation of dermal cells such as fibroblasts. To this end, human plasma gels were synthesized with the addition of increasing concentrations of transglutaminase (TGase), which catalyses the formation of covalent bonds between Lys and Glu residues. These materials were structurally characterized using rheology and texturometry and were found to have good structural resistance and elasticity for fibroblast culture. A remarkable improvement in the mechanical properties of the human plasma gels was detected when the two highest TGase concentrations were tested, which may be interpreted as an increase in the number of covalent and non-covalent bonds formed between the plasma protein chains. Furthermore, a human fibroblast primary culture was seeded on human plasma scaffolds and satisfactorily proliferated at 37 °C. This was verified in the images obtained by optical microscopy (OM) and by scanning electron microscopy (SEM), which confirmed that the structure of this type of material is suitable for the growth and proliferation of dermal fibroblasts.
组织工程是近年来临床医学领域发展迅速的一个分支，特别是因为它作为器官移植的潜在替代品的作用。本研究的主要目的是开发生物相容性材料，以形成细胞外基质（ECM）结构，为真皮细胞（如成纤维细胞）的定居、增殖和分化提供必要条件。为此，在人血浆凝胶中加入了不同浓度的转谷氨酰胺酶（TGase），这种酶可以催化赖氨酸（Lys）和谷氨酸（Glu）残基之间的共价键形成。使用流变学和质构分析对这些材料进行了结构表征，结果发现它们具有良好的结构强度和弹性，适合成纤维细胞培养。当测试两种最高浓度的 TGase 时，人血浆凝胶的机械性能得到了显著改善，这可以解释为血浆蛋白链之间形成的共价和非共价键数量增加。此外，将人原代成纤维细胞接种在人血浆支架上，并在 37°C 下满意地增殖。这通过光学显微镜（OM）和扫描电子显微镜（SEM）获得的图像得到了验证，这些图像证实了这种材料的结构适合于真皮成纤维细胞的生长和增殖。
