Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Textile Engineering, Science and Research Branch, Islamic Azad University, Tehran, 1477893855, Iran.
Sci Rep. 2023 Jan 17;13(1):919. doi: 10.1038/s41598-023-28065-6.
Considering the great potential of egg yolk oil (EYO) in management of burn wounds and superb biological properties of polycaprolactone (PCL) and polyethylene glycol (PEG), hereby, a PCL-PEG-EYO scaffold was developed by electrospinning method for burn healing. The physico-chemical characterizations were performed using SEM, FTIR and contact angle tests. The biological properties of the fabricated scaffolds were evaluated by antibacterial test, in vitro cell culturing, MTT assay and in vivo experiments. The SEM images of PCL-PEG-EYO nanofibers demonstrated a uniform bead-free morphology with 191 ± 61 nm diameter. The fabricated scaffold revealed hydrophilicity with the water contact angel of 77°. No cytotoxicity was observed up to 7 days after cell culturing onto the PCL-PEG-EYO nanofibrous surface. The presence of EYO in the PCL-PEG-EYO scaffold meaningfully improved the cell viability, proliferation and attachment compared to PCL-PEG scaffold. Moreover, the PCL-PEG-EYO scaffolds demonstrated antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa bacteria strain. Finally, a statistically significant enhancement in wound closure, re-epithelialization, angiogenesis and collagen synthesis was observed at the end of 21-day treatment period using PCL-PEG-EYO nanofibrous scaffold. Overall, the PCL-PEG-EYO nanofibrous scaffolds demonstrated a great potential in management of full thickness burn wounds in vivo.
考虑到蛋黄油（EYO）在处理烧伤创面方面的巨大潜力，以及聚己内酯（PCL）和聚乙二醇（PEG）的优异生物学特性，我们通过静电纺丝法开发了一种用于烧伤愈合的 PCL-PEG-EYO 支架。采用 SEM、FTIR 和接触角测试对其理化性质进行了表征。通过抗菌试验、体外细胞培养、MTT 试验和体内实验评价了所制备支架的生物学性能。PCL-PEG-EYO 纳米纤维的 SEM 图像显示出具有 191±61nm 直径的均匀无珠形貌。所制备的支架具有亲水性，水接触角为 77°。细胞培养到 PCL-PEG-EYO 纳米纤维表面 7 天后，未观察到细胞毒性。与 PCL-PEG 支架相比，PCL-PEG-EYO 支架中 EYO 的存在显著提高了细胞活力、增殖和附着。此外，PCL-PEG-EYO 支架对金黄色葡萄球菌和铜绿假单胞菌具有抗菌活性。最后，在 21 天治疗期结束时，使用 PCL-PEG-EYO 纳米纤维支架观察到伤口闭合、再上皮化、血管生成和胶原蛋白合成的统计学显著增强。总体而言，PCL-PEG-EYO 纳米纤维支架在体内全层烧伤创面的治疗中具有巨大的潜力。
