Micheels Patrick, Besse Stéphanie, Sarrazin Didier
J Drugs Dermatol. 2016 Sep 1;15(9):1092-8.
Hyaluronic acid (HA) fillers are commonly used for enhancement of lips, and for softening fine lines and correcting skin depressions.
This study sought to investigate whether the Vycross technology used for Volbella gel resulted in a cohesive gel, as documented in our previous studies with three other HA fillers (Restylane NASHA [Q-MED, Uppsala, Sweden], Esthélis Basic CPM™ [Anteis SA, Geneva, Switzerland], and Juvéderm Ultra 3 using Hylacross technology [Allergan, Irvine, CA, USA]).
The "resistance traction test" and "cohesiveness test" were conducted according to standard methods. Juvéderm Volbella gel was injected into the buttock area, both in the superficial reticular and mid-reticular dermis. Tissue samples were analyzed at days 0, 15, and 90 by histology and immunohistochemistry, and visualized using electron microscopy. For Volbella gel, the same ultrasound devices as previously used were employed.
Prior to staining, Volbella gel presented resistance to spreading, suggesting a certain degree of cohesiveness. When smeared between two slides and following toluidine blue staining, the gel was visible through the microscope in the form of multiple tiny discrete particles, possibly resulting from gel desintegration. At 1/3 dilution with saline serum, Volbella gel disintegrated into several lumps, whereas at 1/1 dilution, Volbella gel appeared more cohesive. Yet when adding one drop 70% ethanol, the gel resembled a poorly defined magma, with numerous small lumps. On ultrasound, Volbella gel was found to leak in the hypodermis. On histological analysis, Volbella gel was visible as pools of variables sizes, particularly in the superficial and mid-reticular dermis, but also hypodermis.
Juvéderm Volbella gel appears to be a gel characterized by low-medium cohesiveness. The study findings, combined with our previous work, show that HA fillers using Vycross technology are not ideally suited for superficial use, unlike HA fillers using CPM technology.
J Drugs Dermatol. 2016;15(9):1092-1098.
透明质酸（HA）填充剂常用于丰唇、软化细纹和矫正皮肤凹陷。
本研究旨在调查用于Volbella凝胶的Vycross技术是否能产生一种具有凝聚力的凝胶，正如我们之前对其他三种HA填充剂（瑞蓝NASHA[Q-MED，瑞典乌普萨拉]、Esthélis Basic CPM™[Anteis SA，瑞士日内瓦]以及采用Hylacross技术的乔雅登极致3[美国艾尔建公司，加利福尼亚州欧文市]）所做研究中所记录的那样。
按照标准方法进行“抗拉伸试验”和“内聚性试验”。将乔雅登Volbella凝胶注射到臀部区域的浅表网状真皮层和网状真皮中层。在第0天、15天和90天时，通过组织学和免疫组织化学分析组织样本，并使用电子显微镜进行观察。对于Volbella凝胶，使用与之前相同的超声设备。
在染色前，Volbella凝胶表现出抗扩散性，表明具有一定程度的内聚性。当涂抹在两张载玻片之间并进行甲苯胺蓝染色后，通过显微镜可看到凝胶呈多个微小离散颗粒的形式，这可能是凝胶解体所致。用盐溶液血清按1/3稀释时，Volbella凝胶分解成几个块状物，而按1/1稀释时，Volbella凝胶看起来内聚性更强。然而，加入一滴70%乙醇后，凝胶类似定义不清的岩浆，有许多小块状物。在超声检查中，发现Volbella凝胶在皮下组织中有渗漏。组织学分析显示，Volbella凝胶呈大小不一的液池状可见，尤其在浅表和网状真皮中层，但皮下组织中也有。
乔雅登Volbella凝胶似乎是一种具有中低内聚性的凝胶。研究结果与我们之前的工作相结合表明，与采用CPM技术的HA填充剂不同，采用Vycross技术的HA填充剂不太适合浅表使用。
《药物皮肤病学杂志》2016年；15(9):1092 - 1098。
