Perumbakkam Sudeep, Hunt Henry D, Cheng Hans H
USDA, ARS, Avian Diseases and Oncology Laboratory, East Lansing, MI 48823, USA Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA.
USDA, ARS, Avian Diseases and Oncology Laboratory, East Lansing, MI 48823, USA.
FEMS Microbiol Ecol. 2016 Dec;92(12). doi: 10.1093/femsec/fiw188. Epub 2016 Sep 7.
Marek's disease (MD) is an important neoplastic disease of chickens caused by Marek ': s disease virus (MDV), a highly oncogenic alphaherpesvirus. In this study using two chicken lines, one resistant and another susceptible to MD, splenic T cells and cecal microbiome were profiled to gain a better understanding of primary differences in these lines. The percent of splenic CD4 T cells were similar regardless of MDV challenge status in both bird lines. In contrast, CD8αα profiles were different (P < 0.005) between chicken lines under naïve status and under MDV challenge, suggesting that CD8αα T cells play a key role in mediating MDV infection. Microbiome composition was different between naïve resistant (Blautia spp.) and susceptible birds (Streptococcus spp.) (P < 0.05) during initial colonization. With MDV challenge, both chicken lines showed lower numbers of beneficial Faecalibacterium spp. and increased number of Lactobacillus spp. Metabolic profiles between naïve chicken types were similar but with MDV challenge, there were differences in metabolism in both chicken lines, with amino acid metabolism impacted in resistant birds and lipid metabolism in susceptible birds. These results provide insights into immune response and potential interplay with the microbiome during infection with an oncogenic virus.
马立克氏病（MD）是由马立克氏病病毒（MDV）引起的鸡的一种重要肿瘤性疾病，MDV是一种高度致癌的α疱疹病毒。在本研究中，使用了两个鸡品系，一个对MD有抗性，另一个对MD敏感，对脾脏T细胞和盲肠微生物群进行了分析，以更好地了解这些品系的主要差异。无论MDV攻击状态如何，两个鸡品系中脾脏CD4 T细胞的百分比相似。相比之下，在未接触MDV状态和MDV攻击状态下，两个鸡品系之间的CD8αα谱不同（P<0.005），这表明CD8αα T细胞在介导MDV感染中起关键作用。在初始定植期间，未接触MDV的抗性鸡（布劳特氏菌属）和易感鸡（链球菌属）之间的微生物群组成不同（P<0.05）。受到MDV攻击后，两个鸡品系中有益的粪杆菌属数量均减少，乳酸杆菌属数量增加。未接触MDV的鸡品系之间的代谢谱相似，但受到MDV攻击后，两个鸡品系的代谢均存在差异，抗性鸡的氨基酸代谢受到影响，易感鸡的脂质代谢受到影响。这些结果为致癌病毒感染期间的免疫反应以及与微生物群的潜在相互作用提供了见解。
