Ergin Ekin, Dogan Arin, Parmaksiz Mahmut, Elçin Ayse E, Elçin Yasar M
Tissue Engineering, Biomaterials & Nanobiotechnology Laboratory, Ankara University Faculty of Science, and Ankara University Stem Cell Institute, Ankara 06100, Turkey.
Curr Pharm Biotechnol. 2016;17(14):1222-1230. doi: 10.2174/1389201017666160809164527.
Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) is a fluorescence based technique which enables the analysis of molecular interactions in biochemical processes. Principle of TR-FRET is based on time-resolved fluorescence (TRF) measurement and fluorescence resonance energy transfer (FRET) between donor and acceptor molecules. To generate FRET signal, donor and acceptor molecules must show spectral overlap and should be in close proximity to each other and display suitable dipole orientation. The specific signal is acquired from molecules of interest via interactions of donor and acceptor molecules. TR-FRET technique is widely used for studying kinase assays, cellular signaling pathways, protein-protein interactions, DNA-protein interactions, and receptor-ligand binding. There are various propriety applications of TR-FRET. Two different sample protocols are summarized in this review.
时间分辨荧光共振能量转移（TR-FRET）是一种基于荧光的技术，可用于分析生化过程中的分子相互作用。TR-FRET的原理基于时间分辨荧光（TRF）测量以及供体和受体分子之间的荧光共振能量转移（FRET）。为了产生FRET信号，供体和受体分子必须表现出光谱重叠，并且彼此靠近并显示出合适的偶极子方向。通过供体和受体分子的相互作用从感兴趣的分子中获取特定信号。TR-FRET技术广泛用于研究激酶测定、细胞信号通路、蛋白质-蛋白质相互作用、DNA-蛋白质相互作用以及受体-配体结合。TR-FRET有各种合适的应用。本综述总结了两种不同的样品方案。
