高固含量下，通过补料分批同步糖化发酵结合分步水解发酵，由碱预处理玉米芯生产富马酸。
Fumaric Acid Production from Alkali-Pretreated Corncob by Fed-Batch Simultaneous Saccharification and Fermentation Combined with Separated Hydrolysis and Fermentation at High Solids Loading.
作者信息
Li Xin, Zhou Jin, Ouyang Shuiping, Ouyang Jia, Yong Qiang
机构信息
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, 210037, China.
Key Laboratory of Forest Genetics and Biotechnology of the Ministry of Education, Nanjing Forestry University, Nanjing, 210037, China.
出版信息
Appl Biochem Biotechnol. 2017 Feb;181(2):573-583. doi: 10.1007/s12010-016-2232-3. Epub 2016 Sep 7.
Production of fumaric acid from alkali-pretreated corncob (APC) at high solids loading was investigated using a combination of separated hydrolysis and fermentation (SHF) and fed-batch simultaneous saccharification and fermentation (SSF) by Rhizopus oryzae. Four different fermentation modes were tested to maximize fumaric acid concentration at high solids loading. The highest concentration of 41.32 g/L fumaric acid was obtained from 20 % (w/v) APC at 38 °C in the combined SHF and fed-batch SSF process, compared with 19.13 g/L fumaric acid in batch SSF alone. The results indicated that a combination of SHF and fed-batch SSF significantly improved production of fumaric acid from lignocellulose by R. oryzae than that achieved with batch SSF at high solids loading.
利用米根霉，通过分离水解发酵（SHF）和补料分批同步糖化发酵（SSF）相结合的方式，研究了在高固含量下从碱预处理玉米芯（APC）生产富马酸的情况。测试了四种不同的发酵模式，以在高固含量下使富马酸浓度最大化。在联合SHF和补料分批SSF工艺中，于38℃从20%（w/v）的APC中获得了最高浓度为41.32 g/L的富马酸，而单独的分批SSF中富马酸浓度为19.13 g/L。结果表明，与高固含量下的分批SSF相比，SHF和补料分批SSF相结合显著提高了米根霉从木质纤维素生产富马酸的产量。
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