Zhang Yide, Khan Aamir A, Vigil Genevieve D, Howard Scott S
Opt Express. 2016 Sep 5;24(18):20862-7. doi: 10.1364/OE.24.020862.
We present a series of experiments that demonstrate a super-sensitive chemical imaging technique based on multiphoton frequency-domain fluorescence lifetime imaging microscopy (MPM-FD-FLIM) that shows a 2× improvement in imaging speed compared to the theoretical limit of conventional MPM-FD-FLIM. Additionally, this technique produces unprecedented sensitivity over a large range of fluorescence lifetimes. These results are achieved through simple modifications to data analysis in a conventional MPM-FD-FLIM microscope and are based on an analytical model describing the signal-to-noise ratio (SNR) of a MPM-FD-FLIM system [J. Opt. Soc. Am. A33, B1 (2016)]. Here we experimentally validate this model.
我们展示了一系列实验，这些实验证明了一种基于多光子频域荧光寿命成像显微镜（MPM-FD-FLIM）的超灵敏化学成像技术，与传统MPM-FD-FLIM的理论极限相比，该技术的成像速度提高了两倍。此外，该技术在大范围的荧光寿命上产生了前所未有的灵敏度。这些结果是通过对传统MPM-FD-FLIM显微镜中的数据分析进行简单修改而实现的，并且基于一个描述MPM-FD-FLIM系统信噪比（SNR）的分析模型[《美国光学学会志》A33，B1（2016年）]。在这里，我们通过实验验证了该模型。
