Gong Y-L, Liu F, Jin H, Xu L, Guo F-F
Department of Pharmacy, College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao, Shandong, China.
Eur Rev Med Pharmacol Sci. 2016 Aug;20(16):3480-9.
Ghrelin had been known to promote gastric motility in human and animals previously. We aim to investigate the role of ghrelin in chemotherapy-induced nausea and vomiting.
In the present study, we observed the changes in food intake, kaolin consumption, body weight, plasma ghrelin concentration and expression of ghrelin and its receptor GHS-R1a in the stomach and nucleus tractus solitaries (NTS) in cisplatin-treated rats, and the effects of ghrelin microinjected into NTS on the discharge activity of gastric distension (GD) responsive neurons and gastric motility were also observed.
Cisplatin induced the decrease in food intake and the increase in kaolin consumption of rats. In addition, mRNA expression of GHS-R1a in the stomach and NTS increased significantly after cisplatin treatment. The discharge activity of GD excited (GD-E) and GD inhibited (GD-I) neurons in cisplatin-treated rats was weaker than that of saline treatment, while ghrelin administration into NTS excited most of GD-E and GD-I neurons. Cisplatin induced the decrease in gastric contraction while ghrelin administrated into NTS promoted the gastric motility significantly. However, the amplitude and frequency of gastric contraction promoted by ghrelin in NTS of cisplatin-treated rats were lower than that of saline treated rats. The effects of ghrelin could be completely blocked by its receptor antagonist BIM28163.
These results indicated that ghrelin in the NTS might participate in the regulation of GD-neurons and gastric motility via its receptor in cisplatin-treated rats.
此前已知胃饥饿素可促进人和动物的胃动力。我们旨在研究胃饥饿素在化疗引起的恶心和呕吐中的作用。
在本研究中，我们观察了顺铂处理的大鼠的食物摄入量、高岭土消耗量、体重、血浆胃饥饿素浓度以及胃和孤束核（NTS）中胃饥饿素及其受体GHS-R1a的表达变化，还观察了向NTS微量注射胃饥饿素对胃扩张（GD）反应性神经元放电活动和胃动力的影响。
顺铂导致大鼠食物摄入量减少，高岭土消耗量增加。此外，顺铂处理后胃和NTS中GHS-R1a的mRNA表达显著增加。顺铂处理的大鼠中GD兴奋（GD-E）和GD抑制（GD-I）神经元的放电活动比生理盐水处理的弱，而向NTS注射胃饥饿素可使大多数GD-E和GD-I神经元兴奋。顺铂导致胃收缩减弱，而向NTS注射胃饥饿素可显著促进胃动力。然而，顺铂处理的大鼠中胃饥饿素促进的胃收缩幅度和频率低于生理盐水处理的大鼠。胃饥饿素的作用可被其受体拮抗剂BIM28163完全阻断。
这些结果表明，在顺铂处理的大鼠中，NTS中的胃饥饿素可能通过其受体参与GD神经元和胃动力的调节。
