瘦素可改善人骨髓基质干细胞的成骨细胞分化。

Leptin improves osteoblast differentiation of human bone marrow stroma stem cells.

作者信息

Xu J-C, Wu G-H, Zhou L-L, Yang X-J, Liu J-T

机构信息

Department of Orthopedics, Xiangyang Hospital, Hubei University of Medicine, Xiangyang, Hubei Province, China.

出版信息

Eur Rev Med Pharmacol Sci. 2016 Aug;20(16):3507-13.

PMID:27608914

Abstract

OBJECTIVE

To study the effects of leptin (LEP) on the osteogenic differentiation of human bone marrow stromal cells (hBMSCs) and to explore the mechanism controlling the process.

MATERIALS AND METHODS

Respectively cultivated the third-generation hBMSCs with 100 ng/ml bone morphogenetic protein (BMP) culture media containing 320, 160, 80 and 40 ng/mL LEP, and regular medium. We administered alkaline phosphatase (ALP) dye (on the 7th day) and mineralized nodules alizarin red (on the 21st day) and tested the ALP activity as well as osteocalcin (OCN) level on 7th, 14th, 21st day in each group to establish the best inducing concentration of LEP. 7 days later, we tested bone differentiation related genes expression in the control, 160 ng/mL LEP and 100 ng/mL BMP groups using RT-PCR.

RESULTS

The activity of ALP and OCN in the 160 ng/mL LEP group after 7, 14 and 21 days was lower than that of the BMP group but higher than that of other groups. However, LEP significantly promoted the expression of bone differentiation related genes, namely, Cbfal, ALP, COL-I and OCN.

CONCLUSIONS

LEP promoted the bone differentiation in hBMSCs by promoting the expression of genes related to bone differentiation.

摘要

目的

研究瘦素（LEP）对人骨髓间充质干细胞（hBMSCs）成骨分化的影响，并探讨其调控机制。

材料与方法

分别用含320、160、80和40 ng/mL LEP的100 ng/ml骨形态发生蛋白（BMP）培养基以及常规培养基培养第三代hBMSCs。在第7天进行碱性磷酸酶（ALP）染色，第21天进行矿化结节茜素红染色，并在每组的第7、14、21天检测ALP活性以及骨钙素（OCN）水平，以确定LEP的最佳诱导浓度。7天后，使用RT-PCR检测对照组、160 ng/mL LEP组和100 ng/mL BMP组中骨分化相关基因的表达。