Zhu Ying-Li, Wang Lin-Yuan, Wang Jing-Xia, Wang Chun, Wang Cheng-Long, Zhao Dan-Ping, Wang Zi-Chen, Zhang Jian-Jun
School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China.
Chin J Nat Med. 2016 Aug;14(8):599-606. doi: 10.1016/S1875-5364(16)30070-X.
Paeonia lactiflora root (baishao in Chinese) is a commonly used herb in traditional Chinese medicines (TCM). Two isomers, paeoniflorin (PF) and albiflorin (AF), are isolated from P. lactiflora. The present study aimed to investigate the protective effects of PF and AF on myelosuppression induced by chemotherapy in mice and to explore the underlying mechanisms. The mouse myelosuppression model was established by intraperitoneal (i.p.) injection of cyclophosphamide (CP, 200 mg·kg(-1)). The blood cell counts were performed. The thymus index and spleen index were also determined and bone morrow histological examination was performed. The levels of tumor necrosis factor-α (TNF-α) in serum and colony-stimulating factor (G-CSF) in plasma were measured by Enzyme-Linked Immunosorbent Assays (ELISA) and the serum levels of interleukin-3 (IL-3), granulocyte-macrophagecolony-stimulatingfactor (GM-CSF), and interleukin-6 (IL-6) were measured by radioimmunoassay (RIA). The levels of mRNA expression protein of IL-3, GM-CSF and G-CSF in spleen and bone marrow cells were determined respectively. PF and AF significantly increased the white blood cell (WBC) counts and reversed the atrophy of thymus. They also increased the serum levels of GM-CSF and IL-3 and the plasma level of G-CSF and reduced the level of TNF-α in serum. PF enhanced the mRNA level of IL-3 and AF enhanced the mRNA levels of GM-CSF and G-CSF in the spleen. PF and AF both increased the protein levels of GM-CSF and G-CSF in bone marrow cells. In conclusion, our results demonstrated that PF and AF promoted the recovery of bone marrow hemopoietic function in the mouse myelosuppression model.
芍药根（中药白芍）是中药中常用的草药。从芍药中分离出两种异构体，芍药苷（PF）和 albiflorin（AF）。本研究旨在探讨 PF 和 AF 对小鼠化疗诱导的骨髓抑制的保护作用，并探索其潜在机制。通过腹腔注射环磷酰胺（CP，200mg·kg(-1)）建立小鼠骨髓抑制模型。进行血细胞计数。还测定胸腺指数和脾脏指数，并进行骨髓组织学检查。采用酶联免疫吸附测定法（ELISA）测定血清中肿瘤坏死因子-α（TNF-α）水平和血浆中集落刺激因子（G-CSF）水平，采用放射免疫测定法（RIA）测定血清白细胞介素-3（IL-3）、粒细胞-巨噬细胞集落刺激因子（GM-CSF）和白细胞介素-6（IL-6）水平。分别测定脾脏和骨髓细胞中 IL-3、GM-CSF 和 G-CSF 的 mRNA 表达蛋白水平。PF 和 AF 显著增加白细胞（WBC）计数并逆转胸腺萎缩。它们还增加了血清中 GM-CSF 和 IL-3 的水平以及血浆中 G-CSF 的水平，并降低了血清中 TNF-α的水平。PF 提高了脾脏中 IL-3 的 mRNA 水平，AF 提高了脾脏中 GM-CSF 和 G-CSF 的 mRNA 水平。PF 和 AF 均增加了骨髓细胞中 GM-CSF 和 G-CSF 的蛋白水平。总之，我们的结果表明 PF 和 AF 在小鼠骨髓抑制模型中促进了骨髓造血功能的恢复。
