Wang Qiang-Song, Gao Teng, Cui Yuan-Lu, Gao Li-Na, Jiang Heng-Li
Research Center of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine , Tianjin , PR China and.
Pharm Biol. 2014 Sep;52(9):1189-95. doi: 10.3109/13880209.2014.880490. Epub 2014 Mar 19.
Paeonia lactiflora Pall. (Ranunculaceae) has been used for more than 1000 years in traditional Chinese medicine for the treatment of gynecological problems, cramp, pain, giddiness, and congestion. Paeoniflorin, monoterpene glycosides isolated from P. lactiflora, possesses a variety of pharmacological activities. However, the pharmacological activity of the pharmacological activity of albiflorin, another main monoterpene glycoside, has not been well studied.
The present study investigated the anti-inflammatory activities of paeoniflorin and albiflorin using models of lipopolysaccharides (LPS) induced RAW 264.7 cells.
Production of nitric oxide (NO) was measured by the Griess colorimetric method. In addition, prostaglandin E2 (PGE2), interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-α) synthesis were analyzed using an enzyme-linked immunosorbent assay (ELISA). The protein expression of cyclooxygenase-2 (COX-2) was detected by a cell-based ELISA. The gene expression levels of inducible nitric oxide synthase (iNOS), COX-2, TNF-α, and IL-6 were detected by quantitative real-time reverse-transcription polymerase chain reaction (real-time RT-PCR).
Compared with the LPS-induced group, the inhibition rates of NO, PGE2, TNF-α, and IL-6 production were 17.61, 27.56, 20.57, and 29.01% by paeoniflorin and 17.35, 12.94, 15.29, and 10.78% by albiflorin. The IC50 values of paeoniflorin and albiflorin on NO production were 2.2 × 10(-4 )mol/L and 1.3 × 10(-2 )mol/L, respectively. The protein expression of COX-2 was reduced by 50.98% with paeoniflorin and 17.21% with albiflorin. The inhibition rates of gene expression of iNOS, COX-2, IL-6, and TNF-α were 35.65, 38.08, 19.72, and 45.19% by paeoniflorin and 58.36, 47.64, 50.70, and 12.43% by albiflorin, respectively.
These results show that albiflorin has similar anti-inflammatory effects to paeoniflorin, which provides new evidence that albiflorin can serve as a new chemical marker for the quality control of Paeoniae Radix and the Chinese Pharmacopoeia can be updated.
芍药（毛茛科）在传统中药中用于治疗妇科问题、痉挛、疼痛、眩晕和充血已有1000多年历史。芍药苷是从芍药中分离出的单萜糖苷，具有多种药理活性。然而，另一种主要单萜糖苷芍药内酯苷的药理活性尚未得到充分研究。
本研究利用脂多糖（LPS）诱导的RAW 264.7细胞模型研究芍药苷和芍药内酯苷的抗炎活性。
采用Griess比色法测定一氧化氮（NO）的生成。此外，使用酶联免疫吸附测定（ELISA）分析前列腺素E2（PGE2）、白细胞介素6（IL-6）和肿瘤坏死因子α（TNF-α）的合成。通过基于细胞的ELISA检测环氧合酶-2（COX-2）的蛋白表达。通过定量实时逆转录聚合酶链反应（实时RT-PCR）检测诱导型一氧化氮合酶（iNOS）、COX-2、TNF-α和IL-6的基因表达水平。
与LPS诱导组相比，芍药苷对NO、PGE2、TNF-α和IL-6生成的抑制率分别为17.61%、27.56%、20.57%和29.01%，芍药内酯苷的抑制率分别为17.35%、12.94%、15.29%和10.78%。芍药苷和芍药内酯苷对NO生成的IC50值分别为2.2×10(-4)mol/L和1.3×10(-2)mol/L。芍药苷使COX-2的蛋白表达降低50.98%，芍药内酯苷使其降低17.21%。芍药苷对iNOS、COX-2、IL-6和TNF-α基因表达的抑制率分别为35.65%、38.08%、19.72%和45.19%，芍药内酯苷的抑制率分别为58.36%、47.64%、50.70%和12.43%。
这些结果表明芍药内酯苷具有与芍药苷相似的抗炎作用，这为芍药内酯苷可作为芍药质量控制的新化学标志物以及更新《中国药典》提供了新证据。
