School of Life Sciences, Jilin University, Changchun 130012, China.
Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, College of Plant Protection, Jilin Agricultural University, Changchun 130118, China.
Cells. 2021 Oct 16;10(10):2776. doi: 10.3390/cells10102776.
Protein hydrolysate injection (PH) is a sterile solution of hydrolyzed protein and sorbitol that contains 17 amino acids and has a molecular mass of 185.0-622.0 g/mol. This study investigated the effect of PH on hematopoietic function in K562 cells and mice with cyclophosphamide (CTX)-induced hematopoietic dysfunction. In these myelosuppressed mice, PH increased the number of hematopoietic cells in the bone marrow (BM) and regulated the concentration of several factors related to hematopoietic function. PH restored peripheral blood cell concentrations and increased the numbers of hematopoietic stem cells and progenitor cells (HSPCs), B lymphocytes, macrophages, and granulocytes in the BM of CTX-treated mice. Moreover, PH regulated the concentrations of macrophage colony stimulating factor (M-CSF), interleukin (IL)-2, and other hematopoiesis-related cytokines in the serum, spleen, femoral condyle, and sternum. In K562 cells, the PH-induced upregulation of hematopoiesis-related proteins was inhibited by transfection with M-CSF siRNA. Therefore, PH might benefit the BM hematopoietic system via the regulation of M-CSF expression, suggesting a potential role for PH in the treatment of hematopoietic dysfunction caused by cancer therapy.
蛋白水解物注射液（PH）是一种无菌的水解蛋白和山梨醇溶液，含有 17 种氨基酸，分子量为 185.0-622.0 g/mol。本研究探讨了 PH 对环磷酰胺（CTX）诱导的造血功能障碍的 K562 细胞和小鼠造血功能的影响。在这些骨髓抑制的小鼠中，PH 增加了骨髓（BM）中造血细胞的数量，并调节了与造血功能相关的几种因子的浓度。PH 恢复了外周血细胞浓度，并增加了 CTX 处理小鼠 BM 中造血干细胞和祖细胞（HSPCs）、B 淋巴细胞、巨噬细胞和粒细胞的数量。此外，PH 调节了血清、脾脏、股骨髁和胸骨中巨噬细胞集落刺激因子（M-CSF）、白细胞介素（IL）-2 和其他与造血相关的细胞因子的浓度。在 K562 细胞中，M-CSF siRNA 的转染抑制了 PH 诱导的与造血相关蛋白的上调。因此，PH 可能通过调节 M-CSF 的表达有益于 BM 造血系统，提示 PH 在治疗癌症治疗引起的造血功能障碍方面具有潜在作用。
