Faculty of Pharmacy, Shaheed Beheshti University of Medical Sciences, Tehran, PO Box 14155-6153, Iran.
Food Chem Toxicol. 2010 Mar;48(3):854-8. doi: 10.1016/j.fct.2009.12.021. Epub 2009 Dec 28.
The protective effects of different concentrations of aqueous extract of Rhus coriaria L. fruit (75 and 100 microg/ml) and also gallic acid (100 microM) as one of its main components were examined against oxidative stress toxicity induced by cumene hydroperoxide (CHP) in isolated rat hepatocytes. Both extract concentrations and gallic acid (100 microM) significantly (P<0.05) protected the hepatocyte against all oxidative stress markers including cell lysis, ROS generation, lipid peroxidation, glutathione depletion, mitochondrial membrane potential decrease, lysosomal membrane oxidative damage and cellular proteolysis. Aqueous extracts of Rhus coriaria L. (75 and 100 microg/ml) were more effective than gallic acid (100 microM) in protecting hepatocytes against CHP induced lipid peroxidation (P<0.05). On the other hand gallic acid (100 microM) acted more effective than aqueous extracts of Rhus coriaria L. (75 and 100 microg/ml) at preventing hepatocyte membrane lysis (P<0.05). In addition H(2)O(2) scavenging effect of both extract concentrations (75 and 100 microg/ml) were determined in hepatocytes and compared with gallic acid (100 microM). Gallic acid (100 microM) was more effective than aqueous extracts of Rhus coriaria L. (75 and 100 microg/ml) at H(2)O(2) scavenging activity (P<0.05).
鼠李皮果实水提物（75 和 100μg/ml）及其主要成分没食子酸（100μM）对过氧化枯烯诱导的分离大鼠肝细胞氧化应激毒性的保护作用。两种提取物浓度和没食子酸（100μM）均显著（P<0.05）保护肝细胞免受所有氧化应激标志物的影响，包括细胞溶解、ROS 生成、脂质过氧化、谷胱甘肽耗竭、线粒体膜电位降低、溶酶体膜氧化损伤和细胞蛋白水解。鼠李皮果实水提物（75 和 100μg/ml）在保护肝细胞免受 CHP 诱导的脂质过氧化方面比没食子酸（100μM）更有效（P<0.05）。另一方面，没食子酸（100μM）在防止肝细胞膜溶解方面比鼠李皮果实水提物（75 和 100μg/ml）更有效（P<0.05）。此外，还在肝细胞中测定了两种提取物浓度（75 和 100μg/ml）的 H 2 O 2 清除作用，并与没食子酸（100μM）进行了比较。没食子酸（100μM）在 H 2 O 2 清除活性方面比鼠李皮果实水提物（75 和 100μg/ml）更有效（P<0.05）。
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