芽孢杆菌属IV类聚羟基脂肪酸酯合酶基因新亚组的克隆与异源表达
Cloning and heterologous expression of a novel subgroup of class IV polyhydroxyalkanoate synthase genes from the genus Bacillus.
作者信息
Mizuno Kouhei, Kihara Takahiro, Tsuge Takeharu, Lundgren Benjamin R, Sarwar Zaara, Pinto Atahualpa, Nomura Christopher T
机构信息
a Department of Creative Engineering , Kitakyushu National College of Technology , Kitakyushu , Japan.
b Department of Innovative and Engineered Materials , Tokyo Institute of Technology , Yokohama , Japan.
出版信息
Biosci Biotechnol Biochem. 2017 Jan;81(1):194-196. doi: 10.1080/09168451.2016.1230006. Epub 2016 Sep 9.
Many microorganisms harbor genes necessary to synthesize biodegradable plastics known as polyhydroxyalkanoates (PHAs). We surveyed a genomic database and discovered a new cluster of class IV PHA synthase genes (phaRC). These genes are different in sequence and operon structure from any previously reported PHA synthase. The newly discovered PhaRC synthase was demonstrated to produce PHAs in recombinant Escherichia coli.
许多微生物都含有合成被称为聚羟基脂肪酸酯（PHA）的可生物降解塑料所需的基因。我们调查了一个基因组数据库，发现了一组新的IV类PHA合酶基因（phaRC）。这些基因在序列和操纵子结构上与之前报道的任何PHA合酶都不同。新发现的PhaRC合酶在重组大肠杆菌中被证明能产生PHA。
Zotero 插件