Kestler H W, Naidu Y N, Kodama T, King N W, Daniel M D, Li Y, Desrosiers R C
New England Regional Primate Research Center, Southborough, MA.
J Med Primatol. 1989;18(3-4):305-9.
Three infectious molecular clones of SIVmac and one of HIV-2 exhibit remarkable variation in their biological properties despite similarities in genome organization and sequence relatedness. Cloned viruses differed in their ability to grow in various cultured cells, in their ability to infect macaques, and in the location of the env stop codon. Sequences from the 3' end predict that at least three of the four clones do not have an intact, functional nef gene. All four cloned viruses yield infectious virus in HUT-78 and all four cloned viruses are cytopathic.
三种猿猴免疫缺陷病毒（SIVmac）的感染性分子克隆以及一种人类免疫缺陷病毒2型（HIV-2）的感染性分子克隆，尽管在基因组结构和序列相关性上存在相似性，但其生物学特性却表现出显著差异。克隆病毒在不同培养细胞中的生长能力、感染猕猴的能力以及包膜（env）终止密码子的位置均有所不同。来自3'端的序列预测，这四个克隆中至少有三个没有完整、功能性的负调控因子（nef）基因。所有四种克隆病毒都能在HUT-78细胞中产生感染性病毒，并且这四种克隆病毒都具有细胞病变效应。
