Lei Yin-Tao, Li Jun-Fu, Jiang Juan, Li Xiao-Guang, Chen Dai-Yun
Department of Stomatology, Affiliated Hospital of Taishan Medical University. Tai'an 271000, China. E-mail:
Shanghai Kou Qiang Yi Xue. 2016 Jun;25(3):314-6.
To explore the expression of p53 up-regulated modulator of apoptosis (PUMA) gene in salivary adenoid cystic carcinoma (SACC) and its clinical significance.
The expressions of PUMA gene and protein were detected in 27 SACC tumor-adjacent tissues (group A) and 27 SACC tumor tissues (group B) with real-time quantitative PCR and Western blot. Statistical analysis was performed using SPSS 19.0 software package.
The expression of PUMA gene in groups A as calibrator was 1，and expression of PUMA gene in group B was 0.57±0.17. The expression of PUMA protein in groups A and B were 0.94±0.12 and 0.36±0.12，respectively. The expressions of PUMA gene and protein were significantly lower in group B than in group A (P<0.05).
The expression of puma gene in SACC tissues is down-regulated, which is negatively correlated with the development of SACC.
探讨凋亡上调调节因子（PUMA）基因在涎腺腺样囊性癌（SACC）中的表达及其临床意义。
采用实时定量PCR和蛋白质印迹法检测27例SACC癌旁组织（A组）和27例SACC肿瘤组织（B组）中PUMA基因及蛋白的表达。使用SPSS 19.0软件包进行统计学分析。
以A组为对照，PUMA基因表达量为1，B组PUMA基因表达量为0.57±0.17。A组和B组PUMA蛋白表达量分别为0.94±0.12和0.36±0.12。B组PUMA基因和蛋白表达均明显低于A组（P<0.05）。
SACC组织中puma基因表达下调，与SACC的发生发展呈负相关。
