完整的中介体-RNA聚合酶II预起始复合物的结构
Structure of a Complete Mediator-RNA Polymerase II Pre-Initiation Complex.
作者信息
Robinson Philip J, Trnka Michael J, Bushnell David A, Davis Ralph E, Mattei Pierre-Jean, Burlingame Alma L, Kornberg Roger D
机构信息
Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA 94158, USA.
出版信息
Cell. 2016 Sep 8;166(6):1411-1422.e16. doi: 10.1016/j.cell.2016.08.050.
Abstract
A complete, 52-protein, 2.5 million dalton, Mediator-RNA polymerase II pre-initiation complex (Med-PIC) was assembled and analyzed by cryo-electron microscopy and by chemical cross-linking and mass spectrometry. The resulting complete Med-PIC structure reveals two components of functional significance, absent from previous structures, a protein kinase complex and the Mediator-activator interaction region. It thereby shows how the kinase and its target, the C-terminal domain of the polymerase, control Med-PIC interaction and transcription.
摘要
通过冷冻电子显微镜以及化学交联和质谱分析，组装并分析了一个完整的、由52种蛋白质组成、分子量为250万道尔顿的中介体 - RNA聚合酶II预起始复合物（Med-PIC）。所得的完整Med-PIC结构揭示了之前结构中不存在的两个具有功能意义的组分，即一个蛋白激酶复合物和中介体 - 激活剂相互作用区域。由此展示了激酶及其靶点——聚合酶的C末端结构域如何控制Med-PIC相互作用和转录。
相似文献
1
Structure of a Complete Mediator-RNA Polymerase II Pre-Initiation Complex.完整的中介体-RNA聚合酶II预起始复合物的结构
Cell. 2016 Sep 8;166(6):1411-1422.e16. doi: 10.1016/j.cell.2016.08.050.
2
Architecture of the RNA polymerase II-Mediator core initiation complex.RNA 聚合酶 II-Mediator 核心起始复合物的结构。
Nature. 2015 Feb 19;518(7539):376-80. doi: 10.1038/nature14229. Epub 2015 Feb 4.
3
Core Mediator structure at 3.4 Å extends model of transcription initiation complex.核心中介体结构在 3.4Å 处延伸转录起始复合物模型。
Nature. 2017 May 11;545(7653):248-251. doi: 10.1038/nature22328. Epub 2017 May 3.
4
Structures of transcription pre-initiation complex with TFIIH and Mediator.与TFIIH和中介体形成的转录前起始复合物的结构
Nature. 2017 Nov 9;551(7679):204-209. doi: 10.1038/nature24282. Epub 2017 Nov 1.
5
Molecular architecture of the human Mediator-RNA polymerase II-TFIIF assembly.人类中介体-聚合酶 II-TFIIF 组装的分子结构。
PLoS Biol. 2011 Mar;9(3):e1000603. doi: 10.1371/journal.pbio.1000603. Epub 2011 Mar 29.
6
[The functional interaction of an RNA polymerase II Rpb11 subunit with the Med18 subunit (Srb5) of the Saccharomyces cerevisiae mediator complex].[酿酒酵母中介体复合物的RNA聚合酶II Rpb11亚基与Med18亚基（Srb5）的功能相互作用]
Bioorg Khim. 2009 Jul-Aug;35(4):572-6. doi: 10.1134/s1068162009040153.
7
Structure of the Mediator head module.中介体头部模块的结构。
Nature. 2012 Dec 20;492(7429):448-51. doi: 10.1038/nature11670. Epub 2012 Oct 31.
8
Structure of the human Mediator-bound transcription preinitiation complex.人源中介体结合的转录起始前复合物结构。
Science. 2021 Apr 2;372(6537):52-56. doi: 10.1126/science.abg3074. Epub 2021 Mar 11.
9
Structure of the human Mediator-RNA polymerase II pre-initiation complex.人类中介体 - RNA 聚合酶 II 起始前复合物的结构。
Nature. 2021 Jun;594(7861):129-133. doi: 10.1038/s41586-021-03555-7. Epub 2021 Apr 26.
10
Architecture of the Mediator head module.中介体头部模块的结构。
Nature. 2011 Jul 3;475(7355):240-3. doi: 10.1038/nature10162.
引用本文的文献
1
Multi-omics and biochemical reconstitution reveal CDK7-dependent mechanisms controlling RNA polymerase II function at gene 5'- and 3' ends.多组学和生化重组揭示了CDK7依赖性机制在基因5'端和3'端控制RNA聚合酶II的功能。
Cell Rep. 2025 Jul 22;44(7):115904. doi: 10.1016/j.celrep.2025.115904. Epub 2025 Jun 25.
2
Identification of the role of MED6 in the development and prognosis of lung adenocarcinoma based on multi-omics profiling.基于多组学分析鉴定MED6在肺腺癌发生发展及预后中的作用
J Cancer. 2025 Apr 13;16(7):2362-2374. doi: 10.7150/jca.110981. eCollection 2025.
3
Filling in missing puzzle pieces in protein structural biology with cross-linking mass spectrometry.利用交联质谱法填补蛋白质结构生物学中缺失的拼图碎片。
QRB Discov. 2024 Dec 27;6:e6. doi: 10.1017/qrd.2024.13. eCollection 2025.
4
Emergent 3D genome reorganization from the stepwise assembly of transcriptional condensates.转录凝聚物逐步组装产生的紧急三维基因组重组。
bioRxiv. 2025 Feb 27:2025.02.23.639564. doi: 10.1101/2025.02.23.639564.
5
Genome-Wide Identification and Expression Analysis of the Mediator Complex Subunit Gene Family in Cassava.木薯中介体复合物亚基基因家族的全基因组鉴定与表达分析
Int J Mol Sci. 2025 Feb 15;26(4):1666. doi: 10.3390/ijms26041666.
6
Multi-omics and biochemical reconstitution reveal CDK7-dependent mechanisms controlling RNA polymerase II function at gene 5'- and 3'-ends.多组学与生化重构揭示了CDK7依赖性机制在基因5'端和3'端对RNA聚合酶II功能的调控。
bioRxiv. 2025 Jan 8:2025.01.08.632016. doi: 10.1101/2025.01.08.632016.
7
Mechanisms of synergistic Mediator recruitment in RNA polymerase II transcription activation revealed by single-molecule fluorescence.单分子荧光揭示RNA聚合酶II转录激活中协同介质募集的机制
bioRxiv. 2024 Dec 11:2024.12.10.627625. doi: 10.1101/2024.12.10.627625.
8
Structures and compositional dynamics of Mediator in transcription regulation.转录调控中介体的结构与组成动力学
Curr Opin Struct Biol. 2024 Oct;88:102892. doi: 10.1016/j.sbi.2024.102892. Epub 2024 Jul 26.
9
Mechanisms and Functions of the RNA Polymerase II General Transcription Machinery during the Transcription Cycle.RNA 聚合酶 II 一般转录机制在转录周期中的机制和功能。
Biomolecules. 2024 Feb 1;14(2):176. doi: 10.3390/biom14020176.
10
Intrinsic protein disorder is insufficient to drive subnuclear clustering in embryonic transcription factors.内在蛋白质无序不足以驱动胚胎转录因子的核内聚集。
Elife. 2024 Jan 26;12:RP88221. doi: 10.7554/eLife.88221.
本文引用的文献
1
Structure of an RNA polymerase II preinitiation complex.RNA聚合酶II起始前复合物的结构。
Proc Natl Acad Sci U S A. 2015 Nov 3;112(44):13543-8. doi: 10.1073/pnas.1518255112. Epub 2015 Oct 19.
2
Molecular architecture of the yeast Mediator complex.酵母中介体复合物的分子结构
Elife. 2015 Sep 24;4:e08719. doi: 10.7554/eLife.08719.
3
Architecture of the Human and Yeast General Transcription and DNA Repair Factor TFIIH.人类和酵母通用转录及DNA修复因子TFIIH的结构
Mol Cell. 2015 Sep 3;59(5):794-806. doi: 10.1016/j.molcel.2015.07.016.
4
The Phyre2 web portal for protein modeling, prediction and analysis.用于蛋白质建模、预测和分析的Phyre2网络门户。
Nat Protoc. 2015 Jun;10(6):845-58. doi: 10.1038/nprot.2015.053. Epub 2015 May 7.
5
Architecture of the RNA polymerase II-Mediator core initiation complex.RNA 聚合酶 II-Mediator 核心起始复合物的结构。
Nature. 2015 Feb 19;518(7539):376-80. doi: 10.1038/nature14229. Epub 2015 Feb 4.
6
Conserved architecture of the core RNA polymerase II initiation complex.核心 RNA 聚合酶 II 起始复合物的保守结构。
Nat Commun. 2014 Jul 10;5:4310. doi: 10.1038/ncomms5310.
7
Subunit architecture and functional modular rearrangements of the transcriptional mediator complex.转录中介体复合物的亚基结构与功能模块重排
Cell. 2014 Jun 5;157(6):1430-1444. doi: 10.1016/j.cell.2014.05.015. Epub 2014 May 29.
8
Redefining the modular organization of the core Mediator complex.重新定义核心中介体复合物的模块化组织。
Cell Res. 2014 Jul;24(7):796-808. doi: 10.1038/cr.2014.64. Epub 2014 May 9.
9
TFIIH phosphorylation of the Pol II CTD stimulates mediator dissociation from the preinitiation complex and promoter escape.TFIIH 对 Pol II CTD 的磷酸化作用刺激中介体从起始前复合物和启动子逃脱中解离。
Mol Cell. 2014 May 22;54(4):601-12. doi: 10.1016/j.molcel.2014.03.024. Epub 2014 Apr 17.
10
Matching cross-linked peptide spectra: only as good as the worse identification.匹配交联肽谱：仅与较差的鉴定结果一样好。
Mol Cell Proteomics. 2014 Feb;13(2):420-34. doi: 10.1074/mcp.M113.034009. Epub 2013 Dec 12.
Zotero 插件