Kato K, Mori K, Katoh N
Nihon Juigaku Zasshi. 1989 Jun;51(3):530-9. doi: 10.1292/jvms1939.51.530.
The isozyme patterns of lactate dehydrogenase (LDH) of bovine milks are different in normal (LDH1 is predominant) and mastitic milks. We surveyed LDH isozymes of mastitic milks, and found that the isozyme patterns could be separated into two groups, mastitic milk A (higher proportions of LDH1,2 and lower proportions of LDH3-5) and mastitic milk B (relative decrease of LDH1 and increase of LDH2-5, particularly LDH3-5). To elucidate the origin of LDH isozymes in the mastitic milks, the isozyme patterns of granulocytes, monocytes, platelets and lymphocytes (T and B cells) were examined. The patterns of granulocytes and lymphocytes were similar to those of mastitic milks A and B, respectively. Sodium dodecyl sulfate polyacrylamide gel electrophoresis also showed the presence of marker proteins of granulocytes and lymphocytes in mastitic milks A and B, respectively. These results suggested that granulocytes and lymphocytes at least partly contributed to the origin of LDH isozymes in the mastitic milks.
正常牛奶（以乳酸脱氢酶1为主）和患乳房炎牛奶的乳酸脱氢酶（LDH）同工酶模式不同。我们对患乳房炎牛奶的LDH同工酶进行了调查，发现同工酶模式可分为两组，即乳房炎牛奶A（乳酸脱氢酶1、2比例较高，乳酸脱氢酶3 - 5比例较低）和乳房炎牛奶B（乳酸脱氢酶1相对减少，乳酸脱氢酶2 - 5增加，尤其是乳酸脱氢酶3 - 5）。为阐明患乳房炎牛奶中LDH同工酶的来源，我们检测了粒细胞、单核细胞、血小板和淋巴细胞（T细胞和B细胞）的同工酶模式。粒细胞和淋巴细胞的模式分别与乳房炎牛奶A和B的模式相似。十二烷基硫酸钠聚丙烯酰胺凝胶电泳也分别显示在乳房炎牛奶A和B中存在粒细胞和淋巴细胞的标记蛋白。这些结果表明，粒细胞和淋巴细胞至少部分促成了患乳房炎牛奶中LDH同工酶的来源。
