Shi Peiqiong, He Zhan, Li Shaojian, An Xuan, Lv Ning, Ghanim Murad, Cuthbertson Andrew G S, Ren Shun-Xiang, Qiu Bao-Li
Department of Entomology, South China Agricultural University, Guangzhou, China.
Key Laboratory of Bio-Pesticide Innovation and Application, Engineering Technology Research Center of Agricultural Pest Biocontrol of Guangdong Province, Guangzhou, China.
PLoS One. 2016 Sep 9;11(9):e0162558. doi: 10.1371/journal.pone.0162558. eCollection 2016.
The whitefly Bemisia tabaci is a cosmopolitan insect species complex that harbors the obligate primary symbiont Portiera aleyrodidarum and several facultative secondary symbionts including Wolbachia, which have diverse influences on the host biology. Here, for the first time, we revealed two different localization patterns of Wolbachia present in the immature and adult stages of B. tabaci AsiaII7 cryptic species. In the confined pattern, Wolbachia was restricted to the bacteriocytes, while in the scattered pattern Wolbachia localized in the bacteriocytes, haemolymph and other organs simultaneously. Our results further indicated that, the proportion of B. tabaci AsiaII7 individuals with scattered Wolbachia were significantly lower than that of confined Wolbachia, and the distribution patterns of Wolbachia were not associated with the developmental stage or sex of whitefly host. This study will provide a new insight into the various transmission routes of Wolbachia in different whitefly species.
烟粉虱是一种世界性的昆虫复合种，其体内携带着专性初级共生菌烟粉虱杆菌以及包括沃尔巴克氏体在内的几种兼性次级共生菌，这些共生菌对宿主生物学有着多样的影响。在此，我们首次揭示了存在于烟粉虱亚洲II7隐种未成熟和成虫阶段的沃尔巴克氏体的两种不同定位模式。在局限模式中，沃尔巴克氏体局限于含菌细胞，而在分散模式中，沃尔巴克氏体同时定位于含菌细胞、血淋巴和其他器官。我们的结果进一步表明，具有分散型沃尔巴克氏体的烟粉虱亚洲II7个体比例显著低于具有局限型沃尔巴克氏体的个体，且沃尔巴克氏体的分布模式与粉虱宿主的发育阶段或性别无关。本研究将为沃尔巴克氏体在不同粉虱物种中的多种传播途径提供新的见解。
