Ciucanu Ionel, Pilat Luminiţa, Ciucanu Cristian I, Şişu Eugen, Dumitraşcu Victor
Department of Chemistry, West University of Timisoara, Strada Pestalozzi 16, RO-300115 Timisoara, Romania.
Faculty of Medicine, 'Victor Babes' University of Medicine & Pharmacy of Timisoara, Piaţa E. Murgu 2, RO-300041 Timisoara, Romania.
Bioanalysis. 2016 Oct;8(20):2147-56. doi: 10.4155/bio-2016-0107. Epub 2016 Sep 9.
We have developed a method for simultaneous monitoring of more biomarkers from three different classes of compounds by simultaneous analysis of neutral monosaccharides, fatty acids (FAs) and cholesterol as their per-O-methylated derivatives from a drop of blood by GC-MS. This work is a development of our previous results about analysis of neutral monosaccharides from a drop of blood.
METHODS & RESULTS: The simultaneous per-O-methylation was obtained by methylation in one step with methyl iodide and NaOH in DMSO. The per-O-methylated derivatives were separated in one chromatogram. The quantitative analysis was reproducible for five monosaccharides, 22 FAs and cholesterol. The results of this method were compared with those of the enzymatic methods using commercial kits.
This method can avoid the saponification of the FA methyl esters and can analyze for the first time simultaneously neutral monosaccharides, FAs and cholesterol from a drop of blood.
我们开发了一种方法，通过气相色谱 - 质谱联用技术，从一滴血中同时分析中性单糖、脂肪酸（FAs）和胆固醇的全 - O - 甲基化衍生物，从而同时监测来自三类不同化合物的更多生物标志物。这项工作是在我们之前关于一滴血中中性单糖分析结果的基础上开展的。
通过在二甲基亚砜中用甲基碘和氢氧化钠一步甲基化实现同时全 - O - 甲基化。全 - O - 甲基化衍生物在一张色谱图中分离。对五种单糖、22种脂肪酸和胆固醇的定量分析具有可重复性。将该方法的结果与使用商业试剂盒的酶法结果进行了比较。
该方法可避免脂肪酸甲酯的皂化反应，并且能够首次同时分析一滴血中的中性单糖、脂肪酸和胆固醇。
