Tianjin Key Laboratory of Aquatic Science and Technology, School of Environmental and Municipal Engineering, Tianjin Chengjian University, Jinjing Road 26#, Tianjin 300384, PR China; School of Environmental Science and Engineering & Pollution Prevention Biotechnology Laboratory of Hebei Province, Hebei University of Science and Technology, Yuhua East Road 70#, Shijiazhuang 050018, PR China.
School of Environmental Science and Engineering & Pollution Prevention Biotechnology Laboratory of Hebei Province, Hebei University of Science and Technology, Yuhua East Road 70#, Shijiazhuang 050018, PR China.
Bioresour Technol. 2016 Nov;220:641-646. doi: 10.1016/j.biortech.2016.08.084. Epub 2016 Aug 24.
This study investigated the rapid start-up of the anaerobic ammonium oxidation (anammox) strategy by inoculating different biomass ratios of denitrifying granular sludge and anammox bacteria. The results demonstrated that two reactors (R1 and R2) were rapidly and successfully started-up on days 25 and 28, respectively, with nitrogen removal rates (NRRs) of 0.70kg/(m(3)·d) and 0.72kg/(m(3)·d) at biomass ratios of 10:1 (R1) and 50:1 (R2). The explanation for rapid start-up was found by examining the effect of five different sludge extracellular polymeric substances (EPS) on the activity of anammox bacteria in the batch experiments. Batch experiments results first demonstrated that the denitrification sludge EPS (DS-EPS) enhanced the anammox bacteria activity the most, and NO2(-)-N, NH4(+)-N removal rates were 1.88- and 1.53-fold higher than the control with optimal DS-EPS volume of 10mL. The rapid start-up strategy makes possible the application of anammox to practical engineering.
本研究通过接种不同比例的反硝化颗粒污泥和厌氧氨氧化菌，考察了厌氧氨氧化策略的快速启动。结果表明，两个反应器（R1 和 R2）分别在第 25 天和第 28 天快速成功启动，生物量比为 10:1（R1）和 50:1（R2）时的氮去除率（NRR）分别为 0.70kg/（m³·d）和 0.72kg/（m³·d）。通过考察批实验中五种不同污泥胞外聚合物（EPS）对厌氧氨氧化菌活性的影响，找到了快速启动的原因。批实验结果首先表明，反硝化污泥 EPS（DS-EPS）对厌氧氨氧化菌活性的促进作用最大，当 DS-EPS 体积为 10mL 时，NO2（-）-N 和 NH4（+）-N 的去除率分别比对照组高 1.88 倍和 1.53 倍。快速启动策略使得厌氧氨氧化技术在实际工程中得到应用成为可能。
