Li Hui-Juan, Peng Dang-Cong, Chen Guo-Yan, Yao Qian, Zhuo Yang, Wang Bin-Bin
School of Municipal and Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
Key Laboratory of Northwest Water Resource, Environment and Ecology, Ministry of Education, Xi'an 710055, China.
Huan Jing Ke Xue. 2017 Jul 8;38(7):2931-2940. doi: 10.13227/j.hjkx.201701037.
An anaerobic sequencing batch reactor(ASBR) inoculated with activated sludge was employed to investigate the start-up of anaerobic ammonium oxidation(ANAMMOX) process and the spatial distribution of extracellular polymeric substances(EPS) in ANAMMOX granules. The results showed that the removal rates of NH-N and NO-N reached more than 99%. The removal rate of total nitrogen(TN) was 89.87%±0.43% and the TN removal load in the ASBR was 1.7 kg·(m·d). The ratios of nitrite consumption and nitrate production to ammonium consumption were 1.32±0.08 and 0.24±0.03, respectively. Besides, the pH and the effluent nitrate concentration in bulk liquid were considered as two simple indicators for rapid diagnosis of ANAMMOX performance. The proteins(PN) was the main component of EPS in ANAMMOX granular sludge. The PN and polysaccharides(PS) in ANAMMOX granules were (59.61±5.64) mg·g and (12.21±2.04) mg·g, respectively, with the ratio of PN/PS reaching approximately 4.88±1.39. -D-glucofuranose and dead cells were distributed in the outermost layer of granules, while living cells, PN, lipids, -glucosamine and α-mannose were distributed throughout the granules and mainly concentrated on the outside of granules. PN and lipids constituted the framework of ANAMMOX granules and ANAMMOX bacteria were embedded among the PN and lipids matrix.
采用接种活性污泥的厌氧序批式反应器（ASBR）研究厌氧氨氧化（ANAMMOX）工艺的启动以及ANAMMOX颗粒中胞外聚合物（EPS）的空间分布。结果表明，NH-N和NO-N的去除率达到99%以上。总氮（TN）去除率为89.87%±0.43%，ASBR中TN去除负荷为1.7 kg·(m·d)。亚硝酸盐消耗与硝酸盐生成量与铵消耗的比值分别为1.32±0.08和0.24±0.03。此外，将pH值和主体液相中的出水硝酸盐浓度视为快速诊断ANAMMOX性能的两个简单指标。蛋白质（PN）是ANAMMOX颗粒污泥中EPS的主要成分。ANAMMOX颗粒中的PN和多糖（PS）分别为(59.61±5.64) mg·g和(12.21±2.04) mg·g，PN/PS比值约为4.88±1.39。β-D-呋喃葡萄糖和死细胞分布在颗粒的最外层，而活细胞、PN、脂质、β-葡萄糖胺和α-甘露糖分布在整个颗粒中且主要集中在颗粒外部。PN和脂质构成了ANAMMOX颗粒的框架，ANAMMOX细菌嵌入在PN和脂质基质中。
