Smith K E, White K A, Kirk D N
School of Biological Sciences, Queen Mary College, London, U.K.
J Steroid Biochem. 1989 Jul;33(1):81-7. doi: 10.1016/0022-4731(89)90361-0.
The 16 alpha-steroid hydroxylating fungus Sepedonium ampullosporum (CMI strain 203 033) transformed progesterone into 16 alpha-hydroxyprogesterone and four other major metabolites which have not been reported previously for this organism, 6 beta-hydroxyprogesterone, 17 alpha-hydroxyprogesterone, 16 alpha-hydroxyandrostenedione and 16-oxotestosterone (16-ketotestosterone). Among the minor metabolites we have been able to identify 15 alpha-hydroxyprogesterone. This compound has not been reported for S. ampullosporum. The conditions used for transformation had comparatively little effect on the relative proportions of products formed, 16 alpha-hydroxyprogesterone always being the predominant metabolite, but had a major effect on the total yields of metabolites isolatable. These findings suggest that one or more constitutive enzyme systems were responsible for the transformations.
16α-甾体羟化真菌壶孢隔孢菌（CMI菌株203 033）将孕酮转化为16α-羟基孕酮以及其他四种主要代谢产物，这四种代谢产物此前尚未见该菌株的报道，分别为6β-羟基孕酮、17α-羟基孕酮、16α-羟基雄烯二酮和16-氧睾酮（16-酮睾酮）。在次要代谢产物中，我们已鉴定出15α-羟基孕酮。该化合物未见关于壶孢隔孢菌的报道。转化所用条件对形成产物的相对比例影响较小，16α-羟基孕酮始终是主要代谢产物，但对可分离代谢产物的总产量有重大影响。这些发现表明，一种或多种组成型酶系统负责这些转化过程。
