Liu Hengquan, Pan Changjiang, Zhou Shijie, Li Junfeng, Huang Nan, Dong Lihua
College of Materials and Chemistry & Chemical Engineering, Chengdu University of Technology, Chengdu 610059, China.
Jiangsu Provincial Key Laboratory for Interventional Medical Devices, Huaiyin Institute of Technology, Huaiyin 223033, China.
Mater Sci Eng C Mater Biol Appl. 2016 Dec 1;69:1175-82. doi: 10.1016/j.msec.2016.08.028. Epub 2016 Aug 12.
Bio-inorganic films and drug-eluting coatings are usually used to improve the hemocompatibility and inhibit restenosis of vascular stent; however, above bio-performances couldn't combine together with single materials. In the present study, we reported a simple approach to fabricate a metal film with the aim of imparting the stent with good blood compatibility and accelerating endothelialization. The films with various ratios of Cu and Ti were prepared through the physical vapor deposition. Phase structure and element composition were investigated by X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS), respectively. The releasing volume of copper ion in Cu/Ti film was determined by immersing test. The hemolysis ratio, platelet adhesion and clotting time were applied to evaluate the hemocompatibility. The proliferative behaviors of endothelial cells and smooth muscle cells under certain copper concentration were investigated in vitro and in vivo. Results indicated that copper-titanium films exhibited good hemocompatibility in vitro; however, the increase of Cu/Ti ratio could lead to increasing hemolysis ratio. Endothelial cells displayed more proliferative than smooth muscle cells when the copper concentration was <7.5μg/ml, however both cells tended to apoptosis to some degree when the copper concentration was increased. The complete endothelialization of the film with low copper in vivo was observed at the 2nd week, indicating that the copper-titanium film with the lower copper concentration could promote endothelialization. Therefore, the inorganic copper-titanium film could be potential biomaterials to improve blood compatibility and accelerating endothelialization of vascular stents.
生物无机薄膜和药物洗脱涂层通常用于改善血管支架的血液相容性并抑制再狭窄；然而，上述生物性能无法通过单一材料实现。在本研究中，我们报道了一种制备金属薄膜的简单方法，旨在赋予支架良好的血液相容性并加速内皮化。通过物理气相沉积制备了不同铜钛比例的薄膜。分别用X射线衍射（XRD）和X射线光电子能谱（XPS）研究了相结构和元素组成。通过浸泡试验测定了铜钛薄膜中铜离子的释放量。应用溶血率、血小板黏附率和凝血时间来评估血液相容性。在体外和体内研究了特定铜浓度下内皮细胞和平滑肌细胞的增殖行为。结果表明，铜钛薄膜在体外表现出良好的血液相容性；然而，铜钛比例的增加会导致溶血率升高。当铜浓度<7.5μg/ml时，内皮细胞比平滑肌细胞表现出更多的增殖，但当铜浓度增加时，两种细胞都有一定程度的凋亡。在第2周观察到体内低铜薄膜的完全内皮化，表明较低铜浓度的铜钛薄膜可以促进内皮化。因此，无机铜钛薄膜可能是改善血管支架血液相容性和加速内皮化的潜在生物材料。
