Bersanetti Patrícia A, Bueno Tatiane L N, Morandim-Giannetti Andreia de A, Nogueira Regina F, Matos Jivaldo R, Schor Paulo
a Departamento de Informática em Saúde , Escola Paulista de Medicina, Universidade Federal de São Paulo , São Paulo , Brazil.
b Departamento de Oftalmologia e Ciências Visuais , Escola Paulista de Medicina, Universidade Federal de São Paulo , São Paulo , Brazil.
Curr Eye Res. 2017 Apr;42(4):528-533. doi: 10.1080/02713683.2016.1214970. Epub 2016 Sep 9.
In this study, we characterized rabbit corneas subjected to corneal cross-linking (CXL) with açaí extract compared with a riboflavin photo-stimulated procedure.
The corneas of the slaughterhouse rabbits were divided into three groups: control, consisting of untreated corneal samples; riboflavin/UVA, where corneas were treated with 0.1% riboflavin photo-stimulated at 365 nm as the standard protocol; and açaí, where the samples were subjected to 4% açaí extract for 0.5-2 h. After the CXL procedure, corneas of the three groups were characterized by analyzing their elastic modulus and thermal denaturation profile.
The elastic modulus at 3% strain showed an approximately threefold increase in the riboflavin/UVA group and 10.5 times in the corneas treated with 4% açaí extract for 2 h, compared with the control group (p < 0.01). The denaturation temperature values of the two groups of crosslinked corneas increased significantly (p < 0.05) and were more pronounced in the açaí group.
The açaí extract was effective in promoting CXL in rabbit corneas as characterized by the different techniques.
在本研究中，我们对用阿萨伊提取物进行角膜交联（CXL）的兔角膜进行了特征分析，并与核黄素光刺激程序进行了比较。
将屠宰场兔子的角膜分为三组：对照组，由未处理的角膜样本组成；核黄素/UVA组，角膜用0.1%核黄素在365nm下进行光刺激，作为标准方案；阿萨伊组，样本用4%阿萨伊提取物处理0.5 - 2小时。在CXL程序后，通过分析三组角膜的弹性模量和热变性曲线对其进行特征分析。
与对照组相比，3%应变下的弹性模量在核黄素/UVA组中显示出约三倍的增加，在用4%阿萨伊提取物处理2小时的角膜中增加了10.5倍（p < 0.01）。两组交联角膜的变性温度值均显著升高（p < 0.05），且在阿萨伊组中更为明显。
如不同技术所表征的那样，阿萨伊提取物在促进兔角膜CXL方面是有效的。
