Fusconi Massimo, Campo Flaminia, Gallo Andrea, Zambetti Giampietro, Martellucci Salvatore, Seccia Annalisa, de Vincentiis Marco
Department of Sensory Organs, "La Sapienza" University of Rome, Rome, Italy.
Department of Sensory Organs, "La Sapienza" University of Rome, Rome, Italy.
J Voice. 2017 Mar;31(2):248.e1-248.e5. doi: 10.1016/j.jvoice.2016.08.002. Epub 2016 Sep 6.
The reported range of involvement of human papillomavirus (HPV) in laryngeal squamous cell carcinoma (SCC) is wide because of the methods used to detect HPV.
A computerized Medline study was carried out using the following as key words: "Papillomavirus Infections"[Mesh] and "Laryngeal Neoplasms"[Mesh].
Studies that were included were written in English and reported results of HPV DNA with RNA in laryngeal SCC.
There were six reported HPV mRNA extraction. Among these studies, Lewis et al reported that out of the 31 cases analyzed, only 2 were HPV DNA+ and of these only 1 was mRNA HPV+ (3%). Halec et al reported 102 cases of which 32 were HPV DNA+ cases and of which only 6 were mRNA+ (5%). Chernock et al reported 76 cases of which 13 were HPV DNA+ cases and of which 4 were mRNA+ (5%). Masand et al reported 8 cases of which 1 was HPV DNA+ case and none was mRNA+. Gheit et al reported 43 cases of which 4 were HPV DNA+ cases and of which 2 were mRNA+ (4%). Castellsagné et al reported 1042 cases of which 59 were HPV DNA+ case and of which 51 were mRNA+ (4.8%) CONCLUSIONS: When determining the role of HPV in laryngeal SCC, evidence of HPV DNA warrants further examination for E6/E7 mRNA as simple assays such as p16 are nonspecific in laryngeal SCC. Further studies of HPV and its role in laryngeal SCC are warranted.
由于用于检测人乳头瘤病毒（HPV）的方法不同，HPV在喉鳞状细胞癌（SCC）中的受累范围报道差异很大。
使用以下关键词进行了计算机化的医学文献数据库检索：“乳头瘤病毒感染”[医学主题词]和“喉肿瘤”[医学主题词]。
纳入的研究均为英文撰写，并报告了喉SCC中HPV DNA与RNA的检测结果。
有6项研究报道了HPV mRNA提取情况。在这些研究中，刘易斯等人报告，在分析的31例病例中，只有2例HPV DNA阳性，其中只有1例mRNA HPV阳性（3%）。哈莱克等人报告了102例病例，其中32例HPV DNA阳性，其中只有6例mRNA阳性（5%）。切尔诺克等人报告了76例病例，其中13例HPV DNA阳性，其中4例mRNA阳性（5%）。马桑德等人报告了8例病例，其中1例HPV DNA阳性，无mRNA阳性病例。盖特等人报告了43例病例，其中4例HPV DNA阳性，其中2例mRNA阳性（4%）。卡斯特尔斯涅等人报告了1042例病例，其中59例HPV DNA阳性，其中51例mRNA阳性（4.8%）。结论：在确定HPV在喉SCC中的作用时，HPV DNA阳性结果需进一步检测E6/E7 mRNA，因为诸如p16等简单检测方法在喉SCC中是非特异性的。有必要对HPV及其在喉SCC中的作用进行进一步研究。
