Liu Peng, Yang Rui, Zuo Zelan
Department of PICU, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China.
Ministry of Education Key Laboratory of Child Development and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing, 400014, China.
BMC Anesthesiol. 2016 Sep 9;16:77. doi: 10.1186/s12871-016-0239-5.
A new rectal cooling device for therapeutic hypothermia (TH) therapy is designed and is applied in TH treatment of SD rats with ischemic-hypoxic brain damage.
Healthy adult SD rats (n = 45) were randomly assigned into four groups: the healthy control group (n = 5), the ischemia and hypoxia group (n = 10), the rectal TH cooling group (n = 18), and the ice blanket TH cooling group (n = 11). The rats in the rectal cooling and ice blanket TH groups received 12 h treatment after hypoxic-ischemic brain damage had been established, while those in the ischemia and hypoxia group did not. Taking the start of TH as the zero point, rats were sacrificed after 24 h and the brain and rectum tissues were sampled for histological analysis.
The TH induction time (37.3 ± 14.7 min) in the rectal cooling group was significantly shorter (F = 4.937, P < 0.05) than that in the ice blanket cooling group (75.6 ± 27.2 min). The HE and NISSL staining results showed that rats in the rectal TH cooling group had significantly decreased (P < 0.01) positive neurons cell count compared to those in ischemia and hypoxia group. In addition, TUNEL staining indicated that the number of apoptotic cells (3.9 ± 1.8 cells / × 400 field) and the apoptosis index (4.4 % ± 1.5) were significantly lower in rectal TH cooling group (P < 0.05) than in ischemia and hypoxia group (23.2 ± 12.1 cells / × 400 field, 26.6 % ± 12.1). Also, no rectal frostbite or inflammatory infiltration was observed in rats in the rectal TH treatment groups.
Our new cooling device realized rapid TH induction in SD rats with ischemic-hypoxic brain damage, inhibited the apoptosis of cells in the hippocampal CAl region, and did not cause histological damage to the rectal tissues.
设计了一种用于治疗性低温（TH）治疗的新型直肠冷却装置，并将其应用于缺血缺氧性脑损伤的SD大鼠的TH治疗。
将45只健康成年SD大鼠随机分为四组：健康对照组（n = 5）、缺血缺氧组（n = 10）、直肠TH冷却组（n = 18）和冰毯TH冷却组（n = 11）。直肠冷却组和冰毯TH组的大鼠在缺氧缺血性脑损伤建立后接受12小时治疗，而缺血缺氧组的大鼠不接受治疗。以TH开始为零点，24小时后处死大鼠，取脑和直肠组织进行组织学分析。
直肠冷却组的TH诱导时间（37.3±14.7分钟）明显短于冰毯冷却组（75.6±27.2分钟）（F = 4.937，P < 0.05）。HE和NISSL染色结果显示，与缺血缺氧组相比，直肠TH冷却组大鼠的阳性神经元细胞计数明显减少（P < 0.01）。此外，TUNEL染色表明，直肠TH冷却组的凋亡细胞数量（3.9±1.8个细胞/×400视野）和凋亡指数（4.4%±1.5）明显低于缺血缺氧组（23.2±12.1个细胞/×400视野，26.6%±12.1）。此外，在直肠TH治疗组的大鼠中未观察到直肠冻伤或炎症浸润。
我们的新型冷却装置在缺血缺氧性脑损伤的SD大鼠中实现了快速TH诱导，抑制了海马CA1区细胞的凋亡，且未对直肠组织造成组织学损伤。
