Department of Pediatric Intensive Care Unit (PICU), Children's Hospital of Chongqing Medical University, Chongqing, China (mainland).
Ministry of Education Key Laboratory of Child Development and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing, China (mainland).
Med Sci Monit. 2020 Feb 4;26:e919680. doi: 10.12659/MSM.919680.
BACKGROUND Previous studies have shown that a neotype rectal cooling device can induce mild hypothermia (MH) in Sprague-Dawley rats with ischemic-hypoxic brain damage (HIBD) and inhibit cell apoptosis in the hippocampal CAl region, and does not cause damage to rectal tissues. The present study aimed to investigate the effect of rectal MH on bacterial translocation (BT) in Sprague-Dawley rats with HIBD. MATERIAL AND METHODS A total of 60 Sprague-Dawley rats were randomly divided into 4 groups: a control group (group C), a normothermia group (group NT), a cooling blanket group (group CB), and a rectal cooling group (group RC). Rats in group CB and group RC received MH using a cooling blanket and rectal cooling device after HIBD model establishment. Then, we measured diamine oxidase (DAO) and D-lactate level separately in groups NT, CB, and RC. Finally, the spleen, liver, and mesenteric lymph nodes were collected for bacterial culture, and rectal tissues were collected for H&E staining. RESULTS The therapeutic outcome was better in Sprague-Dawley rats receiving rectal MH without rectal injury compared to rats in group CB. Escherichia coli (E. coli) was found in MLNs in group RC. E. coli, Proteus vulgaris, Stenotrophomonas maltophilia, and Acinetobacter lwoffii were detected in the rats of groups CB and NT. At 12 h following rectal MH, DAO and D-lactate levels were lower than in group NT. CONCLUSIONS The neotype rectal MH cooling method could be a potential strategy to induce rapid, controllable hypothermia, thus reducing the possibility of inflammatory cell infiltration and BT incidence.
先前的研究表明，新型直肠冷却装置可诱导缺血缺氧性脑损伤（HIBD）的斯普拉格-道利大鼠产生轻度低体温（MH），并抑制海马 CA1 区的细胞凋亡，且不会对直肠组织造成损伤。本研究旨在探讨直肠 MH 对 HIBD 斯普拉格-道利大鼠细菌易位（BT）的影响。
将 60 只斯普拉格-道利大鼠随机分为 4 组：对照组（C 组）、常温组（NT 组）、冷却毯组（CB 组）和直肠冷却组（RC 组）。CB 组和 RC 组在建立 HIBD 模型后使用冷却毯和直肠冷却装置进行 MH。然后，我们分别测量 NT、CB 和 RC 组的二胺氧化酶（DAO）和 D-乳酸水平。最后，收集脾、肝和肠系膜淋巴结进行细菌培养，并采集直肠组织进行 H&E 染色。
与 CB 组相比，接受直肠 MH 治疗的大鼠未发生直肠损伤，治疗效果更好。RC 组的 MLNs 中发现了大肠杆菌（E. coli）。CB 组和 NT 组的大鼠中检测到了大肠杆菌、普通变形杆菌、嗜麦芽窄食单胞菌和洛菲不动杆菌。在直肠 MH 后 12 h，DAO 和 D-乳酸水平低于 NT 组。
新型直肠 MH 冷却方法可能是一种诱导快速、可控性低体温的潜在策略，从而降低炎症细胞浸润和 BT 发生率的可能性。
