Chen Jiaping, Xu Xiaodong, Tao Hu, Wang Yuanyuan, Chen Hongying
College of Life Sciences, Northwest A&F University, No.22 Xinong Road, Yangling, Shaanxi, China.
College of Science, Northwest A&F University, No.22 Xinong Road, Yangling, Shaanxi, China.
Biomol NMR Assign. 2016 Oct;10(2):391-4. doi: 10.1007/s12104-016-9706-7. Epub 2016 Sep 9.
Porcine reproductive and respiratory syndrome virus (PRRSV) is the causative agent of porcine reproductive and respiratory syndrome, a destructive disease of swine. PRRSV has a single strand positive-sense RNA genome which contains at least ten open reading frames, of these, ORF1a and ORF1b encode polyproteins pp1a and pp1ab. Subsequently, pp1a is cleaved into ten nonstructural proteins, including nonstructural protein 7α and 7β (nsp7α and 7β), the internal cleavage products of a conserved nonstructural protein nsp7. Nsp7 plays a role in provoking the humoral immune system into producing anti-nsp7 antibodies which can be highly and persistently expressed in PRRSV-infected swine. However, the functions of nsp7α and 7β remain unknown. Western blot and radioimmunoprecipitation analysis of the two proteins showed that only cleaved nsp7α was detectable and cleaved nsp7β was not detected in the infected cells. Here, we reported the (1)H, (13)C and (15)N resonance assignment of nsp7α from PRRSV as a basis for further structural and functional studies.
猪繁殖与呼吸综合征病毒（PRRSV）是猪繁殖与呼吸综合征的病原体，这是一种对猪具有毁灭性的疾病。PRRSV具有单链正义RNA基因组，其中包含至少十个开放阅读框，其中，ORF1a和ORF1b编码多聚蛋白pp1a和pp1ab。随后，pp1a被切割成十种非结构蛋白，包括非结构蛋白7α和7β（nsp7α和7β），它们是保守非结构蛋白nsp7的内部切割产物。Nsp7在激发体液免疫系统产生抗nsp7抗体方面发挥作用，这些抗体在PRRSV感染的猪中可高度且持续表达。然而，nsp7α和7β的功能仍然未知。对这两种蛋白进行的蛋白质免疫印迹和放射免疫沉淀分析表明，在感染细胞中仅可检测到切割后的nsp7α，而未检测到切割后的nsp7β。在此，我们报道了PRRSV的nsp7α的（1）H、（13）C和（15）N共振归属，作为进一步进行结构和功能研究的基础。
