Protein expression in human cumulus cells as an indicator of blastocyst formation and pregnancy success.

PURPOSE

The goal for the present study was to implement a technique for protein extraction and identification in human cumulus cells (CCs).

METHODS

Forty samples of CCs were collected after ovum pick-up from patients undergoing intracytoplasmic sperm injection (ICSI). Samples were split into the blastocyst group (n = 10), including patients in which all embryos converted into blastocysts, and the non-blastocyst group (n = 10), including patients in which none of the embryos reached the blastocyst stage or the positive-pregnancy (n = 10) and negative-pregnancy group (n = 10). Proteins were extracted and injected into a liquid chromatography system coupled to a mass spectrometer. The spectra were processed and used to search a database.

RESULTS

There were 87 different proteins in samples from the blastocyst and non-blastocyst groups, in which 30 were exclusively expressed in the blastocyst group and 17 in the non-blastocyst group. Among the 72 proteins detected in the pregnancy groups, 19 were exclusively expressed in the positive, and 16 were exclusively expressed in the negative-pregnancy group.

CONCLUSIONS

CC proteomics may be useful for predicting pregnancy success and the identification of patients that should be included in extended embryo culture programs.