Kosteria Ioanna, Anagnostopoulos Athanasios K, Kanaka-Gantenbein Christina, Chrousos George P, Tsangaris George T
Division of Endocrinology, Diabetes and Metabolism, First Department of Pediatrics, University of Athens, Aghia Sophia Children's Hospital, Athens, Greece.
Proteomics Research Unit, Biomedical Research Foundation of the Academy of Athens, Athens, Greece
In Vivo. 2017 May-Jun;31(3):267-283. doi: 10.21873/invivo.11056.
Despite the explosive increase in the use of Assisted Reproductive Technologies (ART) over the last 30 years, their success rates remain suboptimal. Proteomics is a rapidly-evolving technology-driven science that has already been widely applied in the exploration of human reproduction and fertility, providing useful insights into its physiology and leading to the identification of numerous proteins that may be potential biomarkers and/or treatment targets of a successful ART pregnancy. Here we present a brief overview of the techniques used in proteomic analyses and attempt a comprehensive presentation of recent data from mass spectrometry-based proteomic studies in humans, regarding all components of ARTs, including the male and female gamete, the derived zygote and embryo, the endometrium and, finally, the ART offspring both pre- and postnatally.
尽管在过去30年里辅助生殖技术（ART）的使用呈爆发式增长，但其成功率仍不尽人意。蛋白质组学是一门迅速发展的技术驱动型科学，已广泛应用于人类生殖和生育研究，为生殖生理学提供了有益见解，并鉴定出许多可能作为ART成功妊娠潜在生物标志物和/或治疗靶点的蛋白质。在此，我们简要概述蛋白质组学分析中使用的技术，并尝试全面介绍基于质谱的蛋白质组学研究在人类ART所有组成部分方面的最新数据，包括雄性和雌性配子、衍生的受精卵和胚胎、子宫内膜，以及最后ART子代的产前和产后情况。
