Krupčík Jan, Gorovenko Roman, Špánik Ivan, Sandra Pat, Giardina Matthew
Institute of Analytical Chemistry, Faculty of Chemical and Food Technology, STU, Bratislava, Slovakia.
Institute of Analytical Chemistry, Faculty of Chemical and Food Technology, STU, Bratislava, Slovakia.
J Chromatogr A. 2016 Sep 30;1466:113-28. doi: 10.1016/j.chroma.2016.08.032. Epub 2016 Aug 12.
The performances of forward flow fill and flush (FFF) and of reverse flow fill and flush (RFF) in flow modulated comprehensive two-dimensional gas chromatography (GC×GC) using the same volume of the sampling channel have been studied and compared. Sample models include a reference mixture of hydrocarbons at low concentration, a petroleum reformate product and the essential oil of Rosa damascena Miller. The latter samples contain solutes in different concentrations but some up to 30% allowing to study overloading phenomena in detail. For solutes injected at low quantity, the performance of FFF and RFF is similar. For solutes present in a sample at high quantity, RFF guarantees less broadening and spreading resulting in better quantitation.
在流动调制全二维气相色谱（GC×GC）中，使用相同体积的采样通道，对正向流填充和冲洗（FFF）以及反向流填充和冲洗（RFF）的性能进行了研究和比较。样品模型包括低浓度烃类的参考混合物、石油重整产品和大马士革蔷薇精油。后一种样品含有不同浓度的溶质，但有些溶质浓度高达30%，这使得能够详细研究过载现象。对于少量注入的溶质，FFF和RFF的性能相似。对于样品中大量存在的溶质，RFF保证了更少的展宽和扩散，从而实现更好的定量。
