Chen Lei, Liu Lei, Li Caixia, Hu ChengLi, Su Fan, Liu Run, Zeng Mengqi, Zhao Daina, Liu Jiankang, Guo Yurong, Long Jiangang
Center for Mitochondrial Biology and Medicine and Center for Translational Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology and Frontier Institute of Science and Technology, Xi'an Jiaotong University, Xi'an, China.
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, China.
Mol Nutr Food Res. 2017 Mar;61(3). doi: 10.1002/mnfr.201600433. Epub 2017 Jan 31.
Apple pomace polysaccharides (APP), a free radical scavenger, is one of the major compounds derived from apple pomace. However, whether APP has beneficial effects on metabolic disorders is still unknown.
In the present study, water-soluble APP was isolated from the pomace of the locally abundant "Qinguan" apple and chemically characterized. Then, APP was orally administrated to high-fat diet (HFD)-induced obese mice. We found that APP significantly reduced HFD-induced body weight gain and ameliorated HFD-induced hepatic metabolic disorders and oxidative stress. In a palmitate-loaded HepG2 cell model, APP protected the cells from palmitate-induced insulin resistance and loss of viability by suppressing mitochondrial reactive oxygen species and rescuing mitochondrial respiratory function.
Our work suggests that APP, a promising bioactive food component, successfully improved obesity-associated hepatic metabolic disorder, most likely though the activation of hepatic mitochondrial function and the suppression of mitochondria oxidative stress.
苹果渣多糖（APP）是一种自由基清除剂，是从苹果渣中提取的主要化合物之一。然而，APP对代谢紊乱是否具有有益作用仍不清楚。
在本研究中，从当地盛产的“秦冠”苹果的果渣中分离出水溶性APP并对其进行化学表征。然后，将APP口服给予高脂饮食（HFD）诱导的肥胖小鼠。我们发现，APP显著降低了HFD诱导的体重增加，并改善了HFD诱导的肝脏代谢紊乱和氧化应激。在棕榈酸负载的HepG2细胞模型中，APP通过抑制线粒体活性氧并恢复线粒体呼吸功能，保护细胞免受棕榈酸诱导的胰岛素抵抗和活力丧失。
我们的研究表明，APP作为一种有前景的生物活性食品成分，成功改善了肥胖相关的肝脏代谢紊乱，很可能是通过激活肝脏线粒体功能和抑制线粒体氧化应激来实现的。
