Naglot Sarla, Aggarwal Praveen, Dey Sharmistha, Dalal Krishna
Department of Biophysics, All India Institute of Medical Sciences (AIIMS), New Delhi, India.
Department of Emergency Medicine, All India Institute of Medical Sciences (AIIMS), New Delhi, India.
J Clin Lab Anal. 2017 Mar;31(2). doi: 10.1002/jcla.22028. Epub 2016 Sep 12.
Many studies reported for estimating serum YKL-40 using ELISA or RIA methods. This study introduces the plausible utilization of real-time surface plasmon resonance (SPR) technology in investigating the expression of serum YKL-40 protein levels and ELISA method for serum IgE in bronchial asthma.
A commercially available BIAcore 2000 instrument, based on SPR technology, was utilized for assessing serum YKL-40 levels in a control sample size of 45 and active sample size of 97. Antibody immobilization was optimized to obtain the best sensor performance and a sensitive analytic detection. A commercially available ELISA kit was utilized for detecting serum IgE to estimate allergic condition-associated asthma.
The results of SPR technology could distinctly classify with highly statistical significance, the asthma severities by estimating the elevated levels of YKL-40 in blood sera of minute quantities (up to 0.33 ng/ml), and thus differentiates superior utility in comparison with ELISA method. No statistically significant correlation of YKL-40 and IgE was observed.
Serum YKL-40 may be used as a protein marker in classifying asthma severity by applying SPR technology as a reliable, label-free, highly sensitive, and cost-effective tool.
许多研究报道了使用酶联免疫吸附测定（ELISA）或放射免疫分析（RIA）方法来估计血清YKL-40。本研究介绍了实时表面等离子体共振（SPR）技术在研究血清YKL-40蛋白水平表达以及支气管哮喘患者血清IgE的ELISA方法中的合理应用。
使用基于SPR技术的市售BIAcore 2000仪器，对45例对照样本和97例活性样本进行血清YKL-40水平评估。优化抗体固定以获得最佳传感器性能和灵敏的分析检测。使用市售ELISA试剂盒检测血清IgE以评估与过敏相关的哮喘。
SPR技术的结果能够以高度统计学意义清晰地对哮喘严重程度进行分类，通过估计微量血清（高达0.33 ng/ml）中YKL-40水平的升高来实现，因此与ELISA方法相比具有更高的实用性。未观察到YKL-40与IgE之间具有统计学意义的相关性。
通过将SPR技术作为一种可靠、无标记、高度灵敏且经济高效的工具，血清YKL-40可作为哮喘严重程度分类的蛋白质标志物。
