Yamashita Masahiro, Niisato Miyuki, Hanasaka Tomohito, Iwama Noriyuki, Takahashi Tohru, Sugai Tamotsu, Ono Masao, Yamauchi Kohei
1 Department of Pulmonary Medicine, Allergy and Rheumatology, Iwate Medical University School of Medicine , Morioka, Japan .
2 Department of Pathology, Tohoku University Graduate School of Medicine , Sendai, Japan .
Lymphat Res Biol. 2016 Dec;14(4):210-219. doi: 10.1089/lrb.2015.0042. Epub 2016 Sep 12.
Limited information is available regarding the lymphatic vasculature during pneumonia.
To characterize lymphatic vasculatures in autopsied cadavers with pneumonia.
Paraffin-embedded lung tissues obtained from 20 autopsied cadavers with complicated pneumonia and 10 control cadavers without pneumonia were used for immunohistochemical analyses using primary antibodies against podoplanin, vascular endothelial growth factor receptor-3 (VEGFR-3), CD34, vascular endothelial growth factor (VEGF)-C, VEGF-D, CD73, and CD163.
There was no difference in the vascular density of podoplanin usual lymphatics between the individuals with and without pneumonia. In half of the cadavers with pneumonia, however, a network of podoplanin cells lying together in a side-by-side bead-like arrangement appeared along the alveolar septa; however, this was absent in the control cadavers. The podoplanin cells in the network were characterized by a weaker expression of podoplanin, relative to usual lymphatics, and the occasional presence of ductal structures. Although podoplanin cells were not coexpressed with VEGFR-3, a part of the network was connected to CD73 afferent lymphatics. The network showed an intertwined relationship with CD34 capillaries, suggesting that the network represents lymphatic capillaries. The number of CD163 macrophages was significantly increased in individuals with the network than those without the network, while a significant decrease in neutrophils was observed. VEGF-C expressed in CD163 macrophages and type II epithelial cells was observed in the cadavers with the network.
The development of lymphatic capillary networks along the alveolar septa rather than the usual lymphangiogenesis was noted in autopsied individuals with pneumonia.
关于肺炎期间淋巴管系统的信息有限。
对肺炎尸检尸体的淋巴管系统进行特征描述。
从20例患有复杂性肺炎的尸检尸体和10例无肺炎的对照尸体获取石蜡包埋的肺组织，使用抗足板蛋白、血管内皮生长因子受体-3（VEGFR-3）、CD34、血管内皮生长因子（VEGF）-C、VEGF-D、CD73和CD163的一抗进行免疫组织化学分析。
有肺炎和无肺炎个体之间足板蛋白正常淋巴管的血管密度没有差异。然而，在一半的肺炎尸体中，沿着肺泡隔出现了足板蛋白细胞网络，它们以并排珠状排列在一起；然而，对照尸体中没有这种情况。该网络中的足板蛋白细胞相对于正常淋巴管，其足板蛋白表达较弱，且偶尔存在导管结构。虽然足板蛋白细胞不与VEGFR-3共表达，但网络的一部分与CD73传入淋巴管相连。该网络与CD34毛细血管呈交织关系，表明该网络代表毛细淋巴管。有该网络的个体中CD163巨噬细胞数量显著高于无该网络的个体，而中性粒细胞数量显著减少。在有该网络的尸体中观察到CD163巨噬细胞和II型上皮细胞中表达VEGF-C。
在肺炎尸检个体中，观察到沿着肺泡隔而非正常淋巴管生成的毛细淋巴管网络的发育。
