De Martini I, Bianchini D, Schenone A, Cadoni A, Zicca A, Zaccheo D, Mancardi G L
Institute of Clinical Neurology, University of Genova, Italy.
Neurosci Lett. 1989 May 22;100(1-3):331-4. doi: 10.1016/0304-3940(89)90708-8.
T lymphocytes control the extent of the immune reaction by recognizing the antigen in connection with class II histocompatibility surface molecules, coded by genes located on the HLA-D locus. The expression of HLA-DR antigens is confined to a few antigen presenting cells, like lymphocytes and macrophages, which can therefore induce the initial phase of the immune reaction. We report that also Schwann cells (SC) from patients with Charcot-Marie-Tooth disease (CMT), an hereditary disorder of the peripheral nervous system, are able to express HLA-DR antigens. Human SC cultures were carried out from sural nerve biopsies of CMT and normal control cases. Cultures were tested on day 7, 14, 21 and 28, with double immunofluorescence technique using rabbit antiserum anti-S-100 and mouse anti-HLA-DR monoclonal antibody. SC from CMT were HLA-DR positive since the first few days, continuing to express class II antigens for all the duration of the culture. The presence of class II antigens on cultured SC from CMT disease suggests that immune-mediated mechanisms may be relevant in the pathogenesis of this degenerative disorder of the peripheral nervous system.
T淋巴细胞通过识别与II类组织相容性表面分子相关的抗原（由位于HLA - D位点的基因编码）来控制免疫反应的程度。HLA - DR抗原的表达局限于少数抗原呈递细胞，如淋巴细胞和巨噬细胞，因此这些细胞可引发免疫反应的初始阶段。我们报道，来自夏科 - 马里 - 图斯病（CMT，一种遗传性周围神经系统疾病）患者的雪旺细胞（SC）也能够表达HLA - DR抗原。从CMT患者和正常对照病例的腓肠神经活检组织中进行人SC培养。在第7、14、21和28天对培养物进行检测，使用兔抗S - 100抗血清和小鼠抗HLA - DR单克隆抗体采用双重免疫荧光技术。来自CMT患者的SC从最初几天起就是HLA - DR阳性，并在整个培养期间持续表达II类抗原。在CMT病患者培养的SC上存在II类抗原表明免疫介导机制可能与这种周围神经系统退行性疾病的发病机制相关。
