Malacrida Alessio, Maggioni Daniele, Cassetti Arianna, Nicolini Gabriella, Cavaletti Guido, Miloso Mariarosaria
a School of Medicine and Surgery, University of Milan-Bicocca , Monza , Italy.
b Experimental Neurology Unit, University of Milano-Bicocca , Monza , Italy.
Nutr Cancer. 2016 Oct;68(7):1161-70. doi: 10.1080/01635581.2016.1208830. Epub 2016 Aug 11.
Cancer is a leading cause of death worldwide. Despite therapeutic improvements, some cancers are still untreatable. Recently there has been an increasing interest in the use of natural substances for cancer prevention and treatment. Hibiscus sabdariffa (HS) is a plant, belonging to Malvaceae family, widespread in South Asia and Central Africa. HS extract (HSE) used in folk medicine, gained researchers' interest thanks to its antioxidant, anti-inflammatory, and chemopreventive properties. In the present study, we initially assessed HSE effect on a panel of human tumor cell lines. Then we focused our study on the following that are most sensitive to HSE action cell lines: Multiple Myeloma (MM) cells (RPMI 8226) and Oral Squamous Cell Carcinoma (OSCC) cells (SCC-25). In both RPMI 8226 and SCC-25 cells, HSE impaired cell growth, exerted a reversible cytostatic effect, and reduced cell motility and invasiveness. We evaluated the involvement of MAPKs ERK1/2 and p38 in HSE effects by using specific inhibitors, U0126 and SB203580, respectively. For both SCC-25 and RPMI 8226, HSE cytostatic effect depends on p38 activation, whereas ERK1/2 modulation is crucial for cell motility and invasiveness. Our results suggest that HSE may be a potential therapeutic agent against MM and OSCC.
癌症是全球主要的死亡原因之一。尽管治疗方法有所改进，但某些癌症仍然无法治愈。最近，人们对使用天然物质预防和治疗癌症的兴趣日益增加。玫瑰茄（HS）是一种植物，属于锦葵科，在南亚和中非广泛分布。玫瑰茄提取物（HSE）在民间医学中使用，因其抗氧化、抗炎和化学预防特性而引起了研究人员的兴趣。在本研究中，我们首先评估了HSE对一组人类肿瘤细胞系的作用。然后，我们将研究重点放在对HSE作用最敏感的以下细胞系上：多发性骨髓瘤（MM）细胞（RPMI 8226）和口腔鳞状细胞癌（OSCC）细胞（SCC-25）。在RPMI 8226和SCC-25细胞中，HSE均损害细胞生长，发挥可逆的细胞抑制作用，并降低细胞运动性和侵袭性。我们分别使用特异性抑制剂U0126和SB203580评估了MAPKs ERK1/2和p38在HSE作用中的参与情况。对于SCC-25和RPMI 8226细胞，HSE的细胞抑制作用均取决于p38的激活，而ERK1/2的调节对于细胞运动性和侵袭性至关重要。我们的结果表明，HSE可能是一种针对MM和OSCC的潜在治疗剂。
