Hamrita Bechr, Emira Noumi, Papetti Adele, Badraoui Riadh, Bouslama Lamjed, Ben Tekfa Mohamed-Iheb, Hamdi Assia, Patel Mitesh, Elasbali Abdelbaset Mohamed, Adnan Mohd, Ashraf Syed Amir, Snoussi Mejdi
Research Unit UR17ES30 "Virology & Antiviral Strategies", Institute of Biotechnology, University of Monastir, Monastir, Tunisia.
Department of Biology, College of Science, University of Hail, Hail, P.O. Box 2440, Saudi Arabia.
Evid Based Complement Alternat Med. 2022 May 20;2022:1252672. doi: 10.1155/2022/1252672. eCollection 2022.
The aim of this study was to investigate the phytochemical composition of dried Roselle calyx ( L.) using both ethanolic and aqueous extracts. We report the antimicrobial activities against a wide range of bacteria, yeast, and fungi. The antioxidant activities were tested using 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl, and 2-2'-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) radical scavenging assays. We report also for the first time the effect of the swarming motility in PAO1. Our results showed that the tested two extracts were a rich source of phenols, flavonoids, and tannins with different degrees. Additionally, eleven phytoconstituents were identified by LC/MS technique ( acid: 3-caffeoylquinic acid, 5-caffeoylquinic acid, 5-feruloylquinic acid, cyanidin 3-o-glucoside, myricetin, quercetin 7-o-rutinoside, quercetin 3-o-glucoside, delphinidin 3-o-sambubioside, and kaempferol 3-o-p-coumaroyl-glucoside). Also, it was shown that the calyx extract can scavenge 86% of the DPPH radical, while the rate of 53% and 23% of inhibition of the DPPH was obtained only at the concentration of 125 and 50 g/mL, and a small inhibition was made at a concentration of 5 g/mL. Roselle extracts inhibited the growth of the selected microorganisms at low concentrations, while higher concentrations are needed to completely kill them. However, no activity against CVB-3 was recorded for both extracts. In addition, the obtained extracts reduced the swarming motility of at 2.5 mg/ml. The docking simulation showed acceptable binding affinities (up to -9.6 kcal/mol) and interaction with key residues of 1JIJ, 2QZW, and 2UVO. The obtained results highlighted the potential use of Roselle extract as a source of phytoconstituents with promising antimicrobial, antioxidant, and anti-quorum sensing activities.
本研究旨在利用乙醇提取物和水提取物研究干燥玫瑰茄花萼的植物化学成分。我们报告了其对多种细菌、酵母和真菌的抗菌活性。使用2,2-二苯基-1-苦基肼（DPPH）、羟基自由基和2,2'-偶氮二（3-乙基苯并噻唑啉-6-磺酸）自由基清除试验测试了抗氧化活性。我们还首次报告了玫瑰茄花萼提取物对铜绿假单胞菌PAO1群体运动性的影响。我们的结果表明，所测试的两种提取物富含不同程度的酚类、黄酮类和单宁。此外，通过液相色谱/质谱技术鉴定了11种植物成分（酸：3-咖啡酰奎尼酸、5-咖啡酰奎尼酸、5-阿魏酰奎尼酸、矢车菊素3-o-葡萄糖苷、杨梅素、槲皮素7-o-芸香糖苷、槲皮素3-o-葡萄糖苷、飞燕草素3-o-接骨木二糖苷和山奈酚3-o-对香豆酰葡萄糖苷）。此外，结果表明花萼提取物能够清除86%的DPPH自由基，而仅在浓度为125和50μg/mL时对DPPH的抑制率分别为53%和23%，在浓度为5μg/mL时仅有轻微抑制。玫瑰茄提取物在低浓度下可抑制所选微生物的生长，而需要更高浓度才能将其完全杀死。然而，两种提取物均未记录到对柯萨奇病毒B3（CVB-3）的活性。此外，所获得的提取物在浓度为2.5mg/ml时可降低铜绿假单胞菌的群体运动性。对接模拟显示出可接受的结合亲和力（高达-9.6kcal/mol）以及与1JIJ、2QZW和2UVO关键残基的相互作用。所获得的结果突出了玫瑰茄提取物作为具有抗菌、抗氧化和群体感应抑制活性的植物成分来源的潜在用途。
