Gul Mir Zahoor, Chandrasekaran Sambamurthy, K Manjulatha, Bhat Mohd Yasin, Maurya Radheshyam, Qureshi Insaf Ahmed, Ghazi Irfan Ahmad
a Department of Plant Sciences , University of Hyderabad , Gachibowli, Hyderabad , Telangana , India.
b Department of Animal Biology , University of Hyderabad , Gachibowli, Hyderabad , Telangana , India.
Nutr Cancer. 2016 Oct;68(7):1210-24. doi: 10.1080/01635581.2016.1205900. Epub 2016 Aug 11.
ABSTACT Artemisia nilagirica (Clarke) is a widely used medicinal herb in Indian traditional system of medicine. Therefore, the present study was designed to evaluate the effects of A. nilagirica extracts/fractions on inhibition of proliferation and apoptosis in a human monocytic leukemia (THP-1) cell line. The crude extracts (A. nilagirica ethyl acetate extract [ANE] and A. nilagirica methanolic extract [ANA]) showed cytotoxic activity toward THP-1 cells with the IC50 values of 38.21 ± 7.37 and 132.41 ± 7.19 µg/ml, respectively. However, the cytotoxic activity of active fractions (ANE-B and ANM-9) obtained after column chromatography was found to be much more pronounced than their parent extracts. The IC50 values of ANE-B and ANM-9 were found to be 27.04 ± 2.54 µg/ml and 12.70 ± 4.79 µg/ml, respectively, suggesting greater susceptibility of the malignant cells. Cell cycle analysis and terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end-labeling (TUNEL) assay revealed that inhibition of cell growth by A. nilagirica fractions on THP-1 cells was mediated by apoptosis. Active fractions of A. nilagirica increased the expression levels of caspase-3, -7, and poly-ADP-ribose polymerase (PARP), a critical member of the apoptotic pathway. These results suggested that active fractions of A. nilagirica may play a promising role in growth suppression by inducing apoptosis in human monocytic leukemic cells via mitochondria-dependent and death receptor-dependent apoptotic pathways.
摘要 尼尔吉里山艾（Artemisia nilagirica (Clarke)）是印度传统医学中广泛使用的草药。因此，本研究旨在评估尼尔吉里山艾提取物/馏分对人单核细胞白血病（THP-1）细胞系增殖抑制和凋亡的影响。粗提物（尼尔吉里山艾乙酸乙酯提取物[ANE]和尼尔吉里山艾甲醇提取物[ANA]）对THP-1细胞显示出细胞毒性活性，IC50值分别为38.21±7.37和132.41±7.19微克/毫升。然而，柱色谱后获得的活性馏分（ANE-B和ANM-9）的细胞毒性活性比其母体提取物更为显著。发现ANE-B和ANM-9的IC50值分别为27.04±2.54微克/毫升和12.70±4.79微克/毫升，表明恶性细胞更易受影响。细胞周期分析和末端脱氧核苷酸转移酶（TdT）介导的dUTP缺口末端标记（TUNEL）试验表明，尼尔吉里山艾馏分对THP-1细胞生长的抑制是由凋亡介导的。尼尔吉里山艾的活性馏分增加了半胱天冬酶-3、-7和聚ADP核糖聚合酶（PARP）的表达水平，PARP是凋亡途径的关键成员。这些结果表明，尼尔吉里山艾的活性馏分可能通过线粒体依赖性和死亡受体依赖性凋亡途径诱导人单核细胞白血病细胞凋亡，从而在生长抑制中发挥有前景的作用。
