Ren Cong, Wen Zhiqiang, Xu Yan, Jiang Weihong, Gu Yang
State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China; Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China.
Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
Curr Opin Chem Biol. 2016 Dec;35:65-72. doi: 10.1016/j.cbpa.2016.08.024. Epub 2016 Sep 10.
Solventogenic clostridia are native producers of ethanol and many higher alcohols employing a broad range of cheap renewable substrates, such as lignocellulosic materials and C1 gases (CO and CO). These characteristics enable solventogenic clostridia to act as flexible microbial platforms for the production of liquid biofuels. With the rapid development of genetic tools in recent years, the intrinsic intractability of clostridia has been largely overcome, thus, engineering clostridia for production of chemicals and fuels has attracted increasing interests. Here, we provide an overview of recent progress in the production of alcohols based on solventogenic clostridia. Saccharolytic, cellulolytic and gas-fermenting clostridia are discussed, with a special focus on strategies for metabolic engineering to enable and to improve clostridia for the production of higher alcohols.
产溶剂梭菌是乙醇和许多高级醇的天然生产者，它们能利用多种廉价的可再生底物，如木质纤维素材料和C1气体（CO和CO）。这些特性使产溶剂梭菌能够作为生产液体生物燃料的灵活微生物平台。近年来，随着遗传工具的迅速发展，梭菌固有的难处理性已在很大程度上被克服，因此，对梭菌进行工程改造以生产化学品和燃料已引起越来越多的关注。在此，我们概述了基于产溶剂梭菌生产醇类的最新进展。讨论了糖化、纤维素分解和气体发酵梭菌，特别关注代谢工程策略，以使梭菌能够并改进其生产高级醇的能力。
