Singh Namrata, Gupta Sunil, Marwa Naina, Pandey Vivek, Verma Praveen C, Rathaur Sushma, Singh Nandita
Eco-auditing Group, CSIR-National Botanical Research Institute, Lucknow, India.
Plant Ecology and Environmental Science, CSIR-National Botanical Research Institute, Lucknow, India.
Chemosphere. 2016 Dec;164:524-534. doi: 10.1016/j.chemosphere.2016.08.119. Epub 2016 Sep 10.
The present study reports the arsenic (As) tolerance mechanism of bacteria Bacillus aryabhattai (NBRI014). The data explores the intracellular accumulation and volatilization of As from the culture medium after 48 h of exposure to 25,000 mg l arsenate As(V). The study also provides the evidence of presence of ars operon in bacteria, which may have played an important role in reducing As toxicity. Additionally, we found 7 differentially expressed proteins to be up-regulated in bacterial cells upon As exposure which may have role in reducing As toxicity inside bacterial cells. Furthermore, Fourier transform infrared (FTIR) spectroscopic techniques were useful to describe the structural and compositional alterations in bacterial cells after As treatment. It showed the changes in peak positions of the spectrum pattern when NBRI014 was grown in medium containing As, indicating that these functional groups viz. (amino, alkyl halides and hydroxyl) present on bacterial surface, which may be involved in As binding. The above results signify that biotechnological application of the isolate NBRI014 could be helpful in removal of As from polluted sites.
本研究报告了阿氏芽孢杆菌（NBRI014）对砷（As）的耐受机制。该数据探究了在暴露于25000mg/L砷酸盐As（V）48小时后，细菌从培养基中对砷的细胞内积累和挥发情况。该研究还提供了细菌中存在ars操纵子的证据，其可能在降低砷毒性方面发挥了重要作用。此外，我们发现7种差异表达蛋白在砷暴露后在细菌细胞中上调，这可能在降低细菌细胞内的砷毒性中发挥作用。此外，傅里叶变换红外（FTIR）光谱技术有助于描述砷处理后细菌细胞的结构和组成变化。当NBRI014在含砷培养基中生长时，它显示了光谱模式峰值位置的变化，表明这些存在于细菌表面的官能团（氨基、卤代烃和羟基）可能参与了砷的结合。上述结果表明，分离株NBRI014的生物技术应用可能有助于从污染场地去除砷。
