Niehoff Ann-Christin, Grünebaum Jonas, Moosmann Aline, Mulac Dennis, Söbbing Judith, Niehaus Rebecca, Buchholz Rebecca, Kröger Sabrina, Wiehe Arno, Wagner Sylvia, Sperling Michael, von Briesen Hagen, Langer Klaus, Karst Uwe
Institute of Inorganic and Analytical Chemistry, University of Münster, Corrensstr. 30, 48149 Münster, Germany.
Department of Pharmaceutical Technology and Biopharmacy, University of Münster, Corrensstr. 48, 48149 Münster, Germany.
Anal Chim Acta. 2016 Sep 28;938:106-13. doi: 10.1016/j.aca.2016.07.021. Epub 2016 Jul 25.
Limited drug penetration into tumor tissue is a significant factor to the effectiveness of cancer therapy. Tumor spheroids, a 3D cell culture model system, can be used to study drug penetration for pharmaceutical development. In this study, a method for quantitative bioimaging of platinum group elements by laser ablation (LA) coupled to inductively coupled plasma mass spectrometry (ICP-MS) is presented. Different matrix-matched standards were used to develop a quantitative LA-ICP-MS method with high spatial resolution. To investigate drug penetration, tumor spheroids were incubated with platinum complexes (Pt(II)acetylacetonate, cisplatin) and the palladium tagged photosensitizer 5,10,15,20-tetrakis(3-hydroxyphenyl)porphyrin (mTHPP). Distribution and accumulation of the pharmaceuticals were determined with the developed method.
药物向肿瘤组织的渗透受限是影响癌症治疗效果的一个重要因素。肿瘤球体作为一种三维细胞培养模型系统，可用于药物渗透研究以促进药物研发。本研究提出了一种通过激光烧蚀（LA）耦合电感耦合等离子体质谱（ICP-MS）对铂族元素进行定量生物成像的方法。使用不同的基质匹配标准物开发了一种具有高空间分辨率的定量LA-ICP-MS方法。为了研究药物渗透情况，将肿瘤球体与铂配合物（乙酰丙酮铂（II）、顺铂）以及钯标记的光敏剂5,10,15,20-四（3-羟基苯基）卟啉（mTHPP）一起孵育。用所开发的方法测定药物的分布和积累情况。
