Lewińska Anna M, Peuhkuri Ruut H, Rode Carsten, Andersen Birgitte, Hoof Jakob B
Department of Biotechnology and Biomedicine, Technical University of Denmark, Kgs. Lyngby, Denmark.
Danish Building Research Institute, Aalborg University, Copenhagen, SV, Denmark.
J Microbiol Methods. 2016 Nov;130:115-122. doi: 10.1016/j.mimet.2016.09.005. Epub 2016 Sep 9.
Indoor fungi are a worldwide problem causing negative health effects for infected building's occupants and even deterioration of building structures. Different fungal species affect buildings and their inhabitants differently. Therefore, rapid and accurate identification of fungi to the species level is essential for health risk assessment and building remediation. This study focuses on molecular identification of two common indoor fungal genera: Stachybotrys and Chaetomium. This study proposes two new DNA barcode candidates for Stachybotrys and Chaetomium: the gene encoding mitogen activated protein kinase (hogA) and the intergenic region between histone 3 and histone 4 (h3-h4) as well as it introduces a rapid - 3.5h - protocol for direct Stachybotrys and Chaetomium species identification, which bypasses culture cultivation, DNA extraction and DNA sequencing.
室内真菌是一个全球性问题，会对受感染建筑物的居住者造成负面健康影响，甚至导致建筑结构恶化。不同的真菌物种对建筑物及其居住者的影响各不相同。因此，快速准确地将真菌鉴定到物种水平对于健康风险评估和建筑修复至关重要。本研究聚焦于两种常见室内真菌属的分子鉴定：葡萄穗霉属和毛壳菌属。本研究提出了两种用于葡萄穗霉属和毛壳菌属的新DNA条形码候选序列：编码丝裂原活化蛋白激酶的基因（hogA）和组蛋白3与组蛋白4之间的基因间隔区（h3 - h4），并引入了一种快速（3.5小时）方案用于直接鉴定葡萄穗霉属和毛壳菌属物种，该方案绕过了培养、DNA提取和DNA测序步骤。
