在正病毒中鉴定出更多的多样性。
Identification of further diversity among posaviruses.
作者信息
Sano Kaori, Naoi Yuki, Kishimoto Mai, Masuda Tsuneyuki, Tanabe Hitomi, Ito Mika, Niira Kazutaka, Haga Kei, Asano Keigo, Tsuchiaka Shinobu, Omatsu Tsutomu, Furuya Tetsuya, Katayama Yukie, Oba Mami, Ouchi Yoshinao, Yamasato Hiroshi, Ishida Motohiko, Shirai Junsuke, Katayama Kazuhiko, Mizutani Tetsuya, Nagai Makoto
机构信息
Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan.
Kurayoshi Livestock Hygiene Service Center, Kurayoshi, Tottori, 683-0017, Japan.
出版信息
Arch Virol. 2016 Dec;161(12):3541-3548. doi: 10.1007/s00705-016-3048-8. Epub 2016 Sep 12.
Recently, there have been reports of new members of posavirus-like viruses in the order Picornavirales. In this study, using a metagenomics approach, 11 posavirus-like sequences (>7,000 nucleotides) were detected in 155 porcine fecal samples. Phylogenetic analysis revealed that the newly identified virus sequences, together with other posavirus-like viruses, form distinct clusters within the order Picornavirales, composed of eight genogroups and unassigned sequences based on amino acid sequences of the helicase and RNA-dependent RNA polymerase regions, with <40 % and <50 % sequence identity, respectively. We propose further classifications of highly diverse posavirus populations based on newly identified sequences from Japanese pig feces.
最近，有关于小RNA病毒目正杯状样病毒新成员的报道。在本研究中，采用宏基因组学方法，在155份猪粪便样本中检测到11条正杯状样序列（>7000个核苷酸）。系统发育分析表明，新鉴定的病毒序列与其他正杯状样病毒一起，在小RNA病毒目内形成了不同的簇，根据解旋酶和RNA依赖性RNA聚合酶区域的氨基酸序列，这些簇由八个基因组群和未分类序列组成，序列同一性分别<40%和<50%。我们基于从日本猪粪便中新鉴定的序列，对高度多样化的正杯状病毒群体提出了进一步分类。
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