产细菌素的远缘链球菌菌株对大鼠牙齿表面变形链球菌感染及定植的影响

Effect of a bacteriocin-producing strain of Streptococcus sobrinus on infection and establishment of Streptococcus mutans on tooth surfaces in rats.

作者信息

Kitamura K, Masuda N, Kato K, Sobue S, Hamada S

出版信息

Oral Microbiol Immunol. 1989 Jun;4(2):65-70. doi: 10.1111/j.1399-302x.1989.tb00101.x.

DOI:10.1111/j.1399-302x.1989.tb00101.x

PMID:2762016

Abstract

The effect of bacteriocin produced by Streptococcus sobrinus MT6223 on infection and establishment of Streptococcus mutans MT6222 was studied in specific pathogen-free rats. These strains were isolated from a carious lesion of a single subject. S. mutans MT6222 was found to be susceptible to the growth inhibitory action of S. sobrinus MT6223. When simultaneously inoculated into the oral cavity of rats, even a small inoculum (10(5) CFU) of S. sobrinus MT6223 completely inhibited colonization of S. mutans MT6222 on the tooth surface. Also, S. sobrinus MT6223 eliminated S. mutans MT6222 when MT6223 (10(8) CFU) was inoculated 2 days after the inoculation of 10(8) CFU cells of MT6222. Similar results were obtained in dental plaque samples from the tooth surface and the fissures of the upper molars at the end of the experiment. However, when S. sobrinus MT6223 (10(8) CFU) was inoculated 2 weeks after the inoculation of S. mutans MT6222 (10(8) CFU), MT6223 coexisted with MT6222. However, the plaque samples showed that MT6223 inhibited the establishment of MT6222 on smooth surfaces, but not in fissures. In addition, MT6223 protected against subsequent infection with MT6222. However, a nonbacteriocinogenic mutant of S. sobrinus MT6223 did not inhibit the infection and establishment of S. mutans MT6222.

摘要

在无特定病原体的大鼠中研究了嗜热栖热放线菌MT6223产生的细菌素对变形链球菌MT6222感染和定植的影响。这些菌株是从一名受试者的龋损中分离出来的。发现变形链球菌MT6222对嗜热栖热放线菌MT6223的生长抑制作用敏感。当同时接种到大鼠口腔中时，即使是少量接种物（10⁵CFU）的嗜热栖热放线菌MT6223也能完全抑制变形链球菌MT6222在牙齿表面的定植。此外，当在接种10⁸CFU的MT6222细胞2天后接种10⁸CFU的MT6223时，嗜热栖热放线菌MT6223能清除变形链球菌MT6222。在实验结束时，从牙齿表面和上颌磨牙裂隙处采集的牙菌斑样本中也得到了类似结果。然而，当在接种变形链球菌MT6222（10⁸CFU）2周后接种嗜热栖热放线菌MT6223（10⁸CFU）时，MT6223与MT6222共存。然而，牙菌斑样本显示，MT6223在光滑表面抑制MT6222的定植，但在裂隙中则不然。此外，MT6223可预防随后MT6222的感染。然而，嗜热栖热放线菌MT6223的非产细菌素突变体并未抑制变形链球菌MT6222的感染和定植。