Rodoman V E, Laberko L A, Korotaev A L, Zarma A A
Pediatriia. 1989(6):57-61.
The authors describe their own method for rapid determination of urine microflora sensitivity to antibacterial drugs. The method is based on the capacity of the microorganisms (as a totality of particles with the refractive index different from that of the medium) of increasing the optical density of the medium under the conditions of unlimited resources of the nutrition and space at the expense of reproduction in a liquid culture medium. The lack of the optical density increase in the urine sample after addition to it of a certain amount of antibacterial substances evidences the death of the population of the microorganisms and of its sensitivity to the antibacterial drug under study. The method proposed by the authors was compared to those widely used in clinical practice. With special reference to a concrete patient, the results obtained with the authors' method turned out to correlate with those derived with the use of the conventional methods for urine microflora sensitivity to antibacterial drugs (the disc method and the triphenyltetrazolium chloride test). That the final result of the investigation can be obtained after 2--6 hours and low labour intensivity of the method permit the institution of adequate antibiotic therapy within the first day since the patient's admission to the hospital.
作者描述了他们自己快速测定尿液微生物对抗菌药物敏感性的方法。该方法基于微生物（作为一群具有与培养基不同折射率的颗粒）在液体培养基中营养和空间资源无限的条件下，通过繁殖来增加培养基光密度的能力。向尿液样本中加入一定量抗菌物质后光密度没有增加，这表明微生物群体死亡及其对所研究抗菌药物敏感。作者提出的方法与临床实践中广泛使用的方法进行了比较。特别针对一名具体患者，作者方法所得结果与使用传统尿液微生物对抗菌药物敏感性检测方法（纸片法和氯化三苯基四氮唑试验）所得结果相关。该研究最终结果可在2至6小时后获得，且该方法劳动强度低，这使得在患者入院第一天就能开始适当的抗生素治疗。
